Abstract
The bacterial oxidation of high ferrous iron concentrations in batch culture has been studied in a packed bed bioreactor. It has been found that aeration rates from 0.49 to 1.2 VVM did not influence the biofilm oxidation activity during the period of biofilm formation up to 30 g l −1 initial ferrous iron concentration. The contribution of swimming, attached and fixed bacteria to the Fe 2+ oxidation process has been evaluated. Kinetics data showed that the oxidation rate depends on the aeration rate, when the initial Fe 2+ concentration exceeded 30 g l −1. The maximum overall Fe 2+ oxidation rate was 1.8 g l −1 h −1, when the initial ferrous iron concentration was in the range 30 to 45 g l −1.
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