Abstract
Intact red blood cells and red blood cell membranes (ghosts) offer two interesting yet different models to investigate chemical events taking place within the lipid bilayer following exposure of cells to reactive oxygen species. Lipid peroxidation and more specifically oxidation of the glycerol phospholipids that contain esterified polyunsaturated fatty acyl groups such as arachidonate and linoleate, can now be examined directly using techniques of electrospray ionization tandem mass spectrometry for both quantitative and qualitative analysis. Previously, we reported the quantitative analysis of oxidized fatty acids derived from phospholipids or red blood cells and that monohydroxyeicosatetraenoic acids (HETEs) increase significantly as esterified products following treatment with tert-butylhydroperoxide (tBuOOH). Interestingly, four separate epoxyeicosatrienoic acids (EETs) that were isobaric to these HETEs were also found in normal red blood cells and the abundance of these components significantly increased after treatment with tBuOOH (1). Chromatograms have been presented for the HETE and EET molecular species derived from glycerophos-phatidylethanolamine (GPE), but not for another major arachidonate containing glycerophospholipid, glycerphosphatidylcholine (GPC). Hydroperoxy containing fatty acyl groups, likely precursors of the esterified HETE were not detected in this model system.
Published Version
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