Abstract
The use of 1,5-diphenylcarbazide as an electron donor coupled with DPIP 2 2 The abbreviations used in this paper are: DPIP, 2,6-dichlorophenolindophenol; DPC, 1,5-diphenylcarbazide; SC, semicarbazide; DPSC, 1,4-diphenylsemicarbazide; DCMU, 3-(3,4-dichlorophenyl)-1,1-dimethylurea; TX, Triton X-100; Chl, chlorophyll; TSF-1 and TSF-2, the subchloroplast fragments (originally designated PD-10 and P-1, respectively) corresponding to photosystem 1 and photosystem 2, obtained by the action of Triton X-100 on chloroplasts (3,4). reduction allows the detection of photosystem 2 in subchloroplast fragments which have lost the ability to evolve oxygen. Various subchloroplast fragment obtained by detergent treatment of spinach chloroplasts have been assayed in this manner. Photosystem 1 activity was measured as NADP reduction in the presence of ascorbate and DPIP. The best separation of the two photosystems was obtained with Triton X-100 at a concentration of 40 mg Chl/gram detergent.
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