Oxidants and Antioxidants: Ultrastructure and Molecular Biology Protocols

Abstract

Edited by Donald Armstrong, 356 pp, with illus, Totowa, NJ, Humana Press, 2002.The book is edited by Donald Armstrong and is part of a series published by Humana Press under the title Methods in Molecular Biology, with John M. Walker as series editor. As Armstrong outlines in his introduction, the book is the 3rd volume under his editorship dealing with topics relating to oxidative stress status. The 2 other books, titled Free Radical and Antioxidant Protocols and Oxidative Stress Biomarkers and Antioxidant Protocols, have also been published by Humana Press and are protocols books.The present volume, a protocols book as well, is organized into 4 parts (Part I. Techniques for Histological Identification; Part II. Techniques for Subcellular Localization; Part III. Techniques for Molecular Biology; and Part IV. Techniques for In Vivo/Ex Vivo/In Vitro Applications) and contains a total of 36 chapters ranging from 5 to 30 pages. Each chapter is written by an expert (or a group of experts), provides an introduction to the concept of the outlined method(s), and describes in step-by-step detail how to perform the method(s) under discussion. Topics range from cytochemical localization of hydrogen peroxide in biologic tissues or intracellular localization of lipid hydroperoxides to studies of gene expression during oxygen deprivation or the in vivo detection of transition metals and nitrosyl-heme complexes, to name a few.The applications presented in this volume range from straightforward immunochemical procedures to rather sophisticated and expensive techniques such as spectral-spatial electron paramagnetic resonance imaging, x-ray diffraction, and confocal microscopy. Nevertheless, any person working in the field of oxidative stress will appreciate this practical and comprehensive collection of up-to-date molecular and ultrastructural applications for the in vitro and in vivo/ex vivo analysis of free radical and antioxidant reactions.