Oxidant and antioxidant status of patients with sickle cell -β thalassemia in Thi-Qar Province, Iraq

Abstract

BackgroundSickle cell/β thalassemia (HbS/ β thal) is a compound heterozygous state for βS and either β+ thalassemia or β0 thalassemia. In sickle cell/ β0 thalassemia, there is no hemoglobin A, whereas, in sickle cell/ β+thalassemia, a variable amount of hemoglobin A is present. Aim of study was to shed a light on the expected association between sickle cell-thalassemia and oxidative stress.MethodsA total of one hundred and fifty (150) subjects were recruited for this study which consists of hundred (100) sickle cell-thalassemia subjects who 53were males and 47 were females and fifty (50) apparently healthy subjects which served as control who matched by sex and age. The present study is designed to determine (MDA) (malondialdehyde) concentration as a marker for lipid peroxidation and measurement some parameters of antioxidants including: transferrin (Tf), ceruloplasmin (Cp), and albumin (Alb), in patients with Sickle cell/β thalassemia.The means MDA and Alb were increase significant while means TF and CP decrease significant in HbS/ β thal patients as compared to control Also it showed same result in both male and female patients with HbS/ β thal as compared with their healthy counterparts. There were no significant difference between male and female patients in means MDA, CP and Alb except mean TF. The result revealed that no significant difference in means MDA while significant difference in TF, CP and Alb in patient among age group. The means MDA, CP and Alb were not significant difference between male and female patients in each age group while there was found significant difference in mean TF between male and female patient in 1-10 age group only.The increase in the oxidative stress level, especially MDA in sickle cell thalassemia patients compared to healthy individuals, confirms that oxidative stress is involved in the pathogenesis of Sickle cell/β thalassemia disease.