Abstract
The present investigation aimed to uncover the effects of exogenous oxalic acid during the sclerotial formation of Polyporus umbellatus, with an emphasis on determining the content of the endogenic oxalic acid in the fungus. To this end, the oxalic acid content of the vegetative mycelia, sclerotia, culture mediums and sclerotial exudate were measured using High Performance Liquid Chromatography (HPLC). Furthermore, the lipid peroxidation was estimated by detecting thiobarbituric bituric acid reactive substances (TBARS). The results showed that the exogenous oxalic acid caused a delay in sclerotial differentiation (of up to 9 or more days), suppressed the sclerotial biomass and decreased the lipid peroxidation significantly in a concentration-dependent manner. Oxalic acid was found at very low levels in the mycelia and the maltose medium, whereas it was found at high levels in the mycelia and sucrose medium. After sclerotial differentiation, oxalic acid accumulated at high levels in both the sclerotia and the sclerotial exudate. Oxalic acid was therefore found to inhibit P. umbellatus sclerotial formation.
Highlights
Is closely associated with a high oxidative state, and H2O2 accumulation was observed in cell walls or around the organelle membranes of the mycelial cells using transmission electronic microscopy (TEM)[16]
Oxalate crystals were observed during the cultivation of sclerotia under artificial conditions using scanning electronic microscopy (SEM)[26], begging the question: what roles does the endogenous oxalic acid play during P. umbellatus mycelial growth and sclerotial formation? This question sparked our interest in determining the endogenous oxalic acid levels in different cultivation mediums, mycelia, sclerotia and the sclerotial exudate of P. umbellatus
Exogenous oxalic acid caused a proportional inhibition in a concentration-dependent manner in P. umbellatus sclerotial differentiation
Summary
Is closely associated with a high oxidative state, and H2O2 accumulation was observed in cell walls or around the organelle membranes of the mycelial cells using transmission electronic microscopy (TEM)[16]. It is known that in response to oxidative stress, enzymatic and non-enzymatic systems play an important role in preventing significant oxidation-induced damage[17,18]. Endogenous antioxidants such as β -carotene[19,20,21,22], ascorbic acid[19,20,23,24], ascorbate and erythroascorbate[25] have been receiving much research attention. Oxalate crystals were observed during the cultivation of sclerotia under artificial conditions using scanning electronic microscopy (SEM)[26], begging the question: what roles does the endogenous oxalic acid play during P. umbellatus mycelial growth and sclerotial formation? The results from this study will help further our understanding of the biological process of P. umbellatus sclerotial transformation
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