Ovotransferrin possesses SOD-like superoxide anion scavenging activity that is promoted by copper and manganese binding

Abstract

The embryo of oviparous species is confronted by a highly oxidative stress generating as it grows and must rely on effective antioxidant system for protection. Proteins of avian egg albumin have been suggested to play the major redox-modulatory role during embryo development. Recently, we found that ovotransferrin (OTf) undergoes distinct thiol-linked self-cleavage in a redox-dependent process. In this study, we explore that OTf is SOD mimic protein with a potent superoxide anion (O 2 − ) scavenging activity. The O 2 − scavenging activity was investigated using the natural xanthine/xanthine oxidase (X/XOD) coupling system. OTf exhibited O 2 − scavenging activity in a dose-dependent manner and showed remarkably higher scavenging activity than the known antioxidants, ascorbate or serum albumin. The isolated half-molecules of OTf exhibited higher scavenging activity than the intact molecule, whereas the N-lobe showed much greater activity. OTf dramatically quenched the O 2 − flux but had no effect on the urate production in the X/XOD system, indicating its unique specificity to scavenge O 2 − but not oxidase inhibition. Strikingly, metal-bound OTf exhibited greater O 2 − dismutation capacity than the apo-protein, ranging from moderate (Zn 2+-OTf and Fe 2+-OTf) to high (Mn 2+-OTf and Cu 2+-OTf) activity with the Cu 2+-OTf being the most potent scavenger. In a highly sensitive fluorogenic assay, the metal-bound OTf exhibited significant increase in the rate of H 2O 2 production in the X/XOD reaction than the apo-OTf, providing evidence that Zn 2+-, Mn 2+- and Cu 2+-OTf possess SOD mimic activity. This finding is the first to describe that OTf is an O 2 − scavenging molecule, providing insight into its novel SOD-like biological function, and heralding a fascinating opportunity for its potential candidacy as antioxidant drug.