OVOCIDAL ACTIVITY OF CYSTODEZ-ULTRA AGAINST ASCARIS SUUM EGGS IN AN IN VITRO LABORATORY EXPERIMENT

Abstract

The ovocidal activity of various concentrations of Cystodez-ultra and the recommended concentration of Phenol was studied in an experiment on cultivation of Ascaris suum eggs in a thermostat at 26–28 oC in Petri dishes in a humid chamber for 30 days, in each of which 1000 eggs were placed. The first chamber was the control one, where the cultivation took place in saline. A solution containing 3, 4 and 5% concentration of the complex preparation Cystodez-ultra was introduced into the second, third and fourth chambers respectively. A 4% Phenol concentration was introduced into the fifth chamber. After a 24-hour exposure, Ascaris suum eggs were washed three times with distilled water, microscoped to find structural changes, and placed on cultivation. During egg cultivation, aeration was carried out once every two days, and embryogenesis was monitored. The viability of Ascaris suum eggs was determined by their appearance under light microscopy by staining and biological assay. The biological assay for experimental infection of white mice by administration of swine roundworm eggs after cultivation was carried out on 50 mice weighing 18–20 g, that were divided into five groups of 10 mice each. Mice of each group were kept isolated in cages and administered 200 Ascaris suum eggs through a plastic tube, which had previously been cultivated for 32 days and taken from groups 1–5. The complex drug Cystodez-ultra 3% in the in vitro experiment against Ascaris suum eggs showed 93.9% intense-effectiveness. The intense-effectiveness of the test drug in 4 and 5% concentration against swine roundworm eggs was 100%. The base drug Phenol 4% provided 88.8% intense-effectiveness. In the control group, 98% of A. suum eggs had the developed larvae inside. A bioassay on white mice confirmed the high efficacy of Cystodez-ultra against migrating roundworm larvae.