Abstract
Fractions derived from Clerodendrum phlomidis Linn. F. and isolated compounds from chloroform extract were evaluated against Earias vittella Fab. for their ovicidal and oviposition deterrent activities. There were 12 fractions obtained with silica gel column chromatographic techniques using different solvent systems. Among the fractions, fraction 5 recorded maximum ovicidal activity of 68.49% and oviposition deterrent activity of 100% at 1000 ppm concentration followed by 500 ppm concentration. The active fraction was further purified using HPLC. The purified fraction was subjected to UV, 1HNMR, 13CNMR, IR and EI-Mass spectroscopy and their spectral data confirmed the compound pectolinaringenin which belongs to the flavonoids. The compound pectolinaringenin was screened for its ovicidal and oviposition deterrent activities. It showed maximum ovicidal activity of 64.29% and oviposition deterrent activity of 100% at 100 ppm concentration against E. vittella. To the best of our knowledge, this is the first report of fractions and isolated compounds from C. phlomidis evaluated against E. vittella for their ovicidal and oviposition deterrent activities. The results from the present investigation clearly suggest that the active fraction or the isolated compound could be used to develop a novel pesticidal formulation to control economically important agricultural pests.
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