Abstract
There is growing research focusing on endothelial cells as separate units of the blood-brain barrier (BBB), and on the complex relationships between different types of cells within a neurovascular unit. To conduct this type of studies, researches use vastly different in vitro BBB models. The main objective of such models is to study the BBB permeability for different molecules, and to advance the current level of understanding the mechanisms of disease and to develop methods of targeted therapy for the central nervous system. The analysis of the existing Abstract in vitro BBB models and their advantages/disadvantages was conducted using the clinical trial data obtained in Russian/foreign countries. In this review, the authors highlight the most relevant assessment parameters and propose a unified classification of in vitro BBB models. According to the performed analysis, there is a tendency to move from 2D BBB models based on semipermeable inserts to 3D BBB spheroid and microfluidic organ-on-chip models. Moreover, the use of human induced pluripotent stem cells instead of animal primary cells will make it possible to reliably scale the results obtained in vitro to conditions in vivo.
Highlights
Проведен анализ имеющихся на сегодняшний день данных исследований гематоэнцефалического барьера (ГЭБ) in vitro
Салмина Алла Борисовна, доктор медицинских наук, Salmina Alla B., MD, PhD, Professor, Leading Researcher профессор главный научный сотрудник научно-исследова- at the Research Institute of Molecular Medicine and тельского института молекулярной медицины и патобио- Pathobiochemistry, Federal State Budgetary Educational химии федерального государственного бюджетного обра- Institution of Higher Education “Krasnoyarsk State Medical зовательного учреждения высшего образования «Красно- University named after Professor V.F
Voino-Yasenetsky” ярский государственный медицинский университет имени of the Ministry of Healthcare of the Russian Federation, профессора В.Ф
Summary
Проведен анализ имеющихся на сегодняшний день данных исследований гематоэнцефалического барьера (ГЭБ) in vitro. Описаны предпосылки возникновения и генезис клеточных моделей ГЭБ в мировой научной практике и методы оценки их компетентности. Предложены классификация моделей ГЭБ in vitro, сравнительная таблица преимуществ и недостатков каждой модели и возможные области их применения; проанализированы современные тренды, проблемы и перспективы использования клеточных моделей ГЭБ
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