Abstract
BackgroundPost-transcriptional methylation modifications, including 5-methylcytosine (m5C) modification, are closely related to the tumorigenesis of cancers. However, the mRNA profile of m5C modification in hepatocellular carcinoma (HCC) is unknown.MethodsMethylated RNA immunoprecipitation sequencing was performed to identify m5C peaks on mRNA of human HCC tissues and adjacent tissues, and differences in m5C between the two groups were analyzed. In addition, we conducted a bioinformatics analysis to predict the function of specific methylated transcripts.ResultsWe found that there was a noticeable difference in m5C between HCC and paired non-tumor tissues, suggesting that m5C could play a role in the pathogenesis of HCC. In addition, analyses of gene ontology and the Kyoto Encyclopedia of Genes and Genomes showed that the unique distribution pattern of mRNA m5C in HCC was associated with a wide range of cellular functions.ConclusionsOur results revealed different distribution patterns of m5C in HCC and adjacent tissues and provided new insights into a novel function of m5C RNA methylation of mRNA in HCC progression.
Highlights
Post-transcriptional methylation modifications, including 5-methylcytosine (m5C) modification, are closely related to the tumorigenesis of cancers
Li et al found that inhibiting the generation of YTHDTF2, which is an m6A reader protein, blocked anti-miR-145-enhanced proliferation, suggesting that miR-145 suppresses the proliferation of hepatocellular carcinoma (HCC) cells by regulating m6A reading [20, 21]. 5-methylcytosine (m5C), which is another post-transcriptional RNA modification, has been identified in stable and highly abundant tRNAs, rRNAs, and mRNAs [22,23,24]
Our findings suggest a possible association between HCC and m5C in mRNA and predict possible functional changes caused by this difference in m5C
Summary
Post-transcriptional methylation modifications, including 5-methylcytosine (m5C) modification, are closely related to the tumorigenesis of cancers. Epigenetic dysregulation plays a critical role in the initiation and progression of cancer, and post-transcriptional modifications, such as RNA methylation, have attracted the attention of many researchers [13]. Studies have shown that m5C modification is necessary for the stable and efficient translation of tRNA and plays an important role in rRNA processing, structuring, and translation [26,27,28,29]. This modification has conservative, tissue-specific, and dynamic characteristics in mammalian transcriptomes [25]. The quantity, distribution, and functions of m5C in HCC are still unclear
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