Overproduction of valuable methoxylated flavones in induced tetraploid plants of Dracocephalum kotschyi Boiss

Aa Zahedi,H Parastar Shahri,E Dehghan,S Rostami,B Hosseini,M Fattahi,Z Moghaddamfar

doi:10.1055/s-0034-1382522

Abstract

Overproduction of valuable methoxylated flavones in induced tetraploid plants of Dracocephalum kotschyi Boiss

Highlights

Ploidy manipulation is considered an efficient method to increase production potential of medicinally important compounds
Putative tetraploids were selected by morphological and microscopic characteristics and their ploidy level was confirmed by flow cytometry analysis and chromosome counting
Determination of ploidy level Flow cytometry analysis Flow cytometry analysis of diploid control plants showed a peak at channel 30, related to the G1 of diploid plants (Figure 1a), while the corresponding peak of tetraploids was at about 60 (Figure 1c)

Summary

Introduction

Ploidy manipulation is considered an efficient method to increase production potential of medicinally important compounds. Recent pharmacological studies have confirmed presence of several methoxylated flavonoids with anti-cancer properties (Jahaniani et al 2005; Moghaddam et al 2012) and inhibitory effects on the lectin-induced cellular immune response in this plant (Faham et al 2008). Flavonoides include over 4000 structurally related compounds existing in nature either as free aglycones or glycosidic conjugates and are generally classified according to their chemical structures into flavones, flavanones, flavanols, flavonols and anthocyanidins (Middleton et al 2000). This diversity of structural patterns has made flavonoids a rich source of compounds with potential anticancer properties. Recent pharmacological studies suggested some methoxylated flavonoids (Xanthomicrol and Calycopterin) of D. kotschyi with anti-cancer properties (Jahaniani et al 2005; Moghaddam et al 2012)

Methods

Results

Conclusion