Abstract
Considering the nutritional values of Mentha viridis. L and Aloe veraplants, these plants can be utilized for the production of alternative cultivation media. The cost of artificial culture media is very high, and some components may be unavailable. Use of the plant-based culture media would drastically reduce the expense of the synthetic media. Fifteen bacterial isolates were isolated from Aloe vera rhizosphere, nine bacterial and nine actinomycetes isolates were isolated from Mentha viridis rhizosphere both cultivated in Sirs EL- Layan, El-Menoufia governorate, Egypt. In-vitroscreening was done for the production of indole acetic acid (IAA) and phosphorus solubilization. Results revealed that bacterial isolate No. MB4 produced a high amount of IAA(36.51 μg/ml) on the Mentha-based culture medium, No. A6 showed maximum IAA production (16.25μg/ml) on the Aloe vera-based culture medium and isolate No. MA6 was efficient in phosphorus solubilization (867.85μg/ml) that was isolated from the Mentha viridis. L rhizosphere.16s rRNA analysis of these isolates revealed they are (Pseudomonas monteilii strain CIP 104883, Streptomyces rochei strain DW3 and Kosakonia radicincitans strain DSM 16656 respectively.
Highlights
Results revealed that bacterial isolate No MB4 produced a high amount of indole-acetic acid (IAA)(36.51 μg/ml) on the Mentha-based culture medium, No A6 showed maximum IAA production (16.25μg/ml) on the Aloe vera-based culture medium and isolate No MA6 was efficient in phosphorus solubilization (867.85μg/ml) that was isolated from the Mentha viridis
Plant-based culture medium is a of selected strains screened by antagonistic activity against pathogenic fungi revealed that Pseudomonas monteilii strain CIP 104883 had shown maximum zone of inhibition against Rhizoctonia solani (19 mm) while Streptomyces rochei and Kosakonia radicincitans showed highly antifungal activity against Fusarium solani (18 mm and 11mm zone of inhibition, respectively)
Youssef et al (2015) who isolated distinctive colonies of rhizobacteria associated with the roots of Aloe vera developed on agar plates of plant-based culture media, that prepared from diluted juices (1:20, v/v) of A. vera
Summary
A total of fifteen bacterial isolates were isolated from Aloe vera rhizosphere on Aloe vera-based culture medium with different dilutions 1:10, 1:20, 1:40, 1:80 and 1:100 v/v (juice or sap: distilled water, v/v) (Plate 1a-d and Plate 2a),these results are harmony with. Nine bacterial and nine actinomycetes isolates were rhizosphere of M. viridis were developed on agar plates of Mentha-based culture media prepared from diluted juices (1:10, 1:20, 1:40, 1:80 and 1:100 v/v) of M. viridis (Plate 2b). The results showed that actinomycetes isolate (Gram-positive) developed on Mentha-based medium rather than Aloe vera-based medium This may occur due to the presence of steroid, triterpenoid, flavonoid, phenol, tannin, alkaloid, saponin and acid in the Aloe vera juice responsible for its antibacterial activity. An earlier report suggested that a large number of Aloe vera extracts to be active against Gram-positive bacteria McCutcheon et al (1992), probably due to the absence of the outer of the bacterial cell wall
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
