Abstract
Fur mutants FPA12 and FF13 of strains Pseudomonas aeruginosa PAO1 and Fe10, respectively, were prepared and their production of pyoverdin evaluated. The strains were cultivated in stirred bioreactor in iron-deficient and iron-supplemented medium containing Casamino acids (CA) or succinate as a source of carbon and energy. When the pyoverdin production rate reached its maximum, the demand of iron-depleted cultures for O2 was decreased. Mutant FF13 overproduced pyoverdin in both iron-depleted (862 mg l−1) and iron-supplemented (428 mg l−1) CA medium and could also be used to produce pyoverdin when grown in a conventional stirred tank fermenter.
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