Overproduction of lipase with staphylococcus carnosus (pLipPS1) under modified gravity in a centrifugal field bioreactor

Abstract

AbstractFermentation under modified gravity could be of interest in application to (a) increasing productivity of growth and growth linked production with microorganisms at high cell densities and (b) increasing the productivity of highly viscous pseudoplastic polysaccharide fermentation. In both cases, higher oxygen transfer rates in centrifugal fields result in higher productivities since these fermentations are usually oxygen limited. A further aspect of fermentation under increased gravity is the reduction of foam since foam coalescence time decreases with acceleration number. On the other hand, under microgravity, shear reduction would allow growth and production even for very shear sensitive organisms. In order to carry out fermentations under modified gravity, a special type of fermenter–the centrifugal field bioreactor CFBR–has been developed at the Institute of Chemical Engineering (Head: Prof. Mersmann) of the Technical University of Munich. For the first time, exoprotein biosynthesis of lipase with S. carnosus has been carried out under sterile and controlled conditions in this novel bioreactor, in presence of increased mass forces.