Abstract
Plasma membrane intrinsic proteins (PIPs) are a subfamily of aquaporin proteins located on plasma membranes where they facilitate the transport of water and small uncharged solutes. PIPs play an important role throughout plant development, and in response to abiotic stresses. Jojoba (Simmondsia chinensis (Link) Schneider), as a typical desert plant, tolerates drought, salinity and nutrient-poor soils. In this study, a PIP1 gene (ScPIP1) was cloned from jojoba and overexpressed in Arabidopsis thaliana. The expression of ScPIP1 at the transcriptional level was induced by polyethylene glycol (PEG) treatment. ScPIP1 overexpressed Arabidopsis plants exhibited higher germination rates, longer roots and higher survival rates compared to the wild-type plants under drought and salt stresses. The results of malonaldehyde (MDA), ion leakage (IL) and proline content measurements indicated that the improved drought and salt tolerance conferred by ScPIP1 was correlated with decreased membrane damage and improved osmotic adjustment. We assume that ScPIP1 may be applied to genetic engineering to improve plant tolerance based on the resistance effect in transgenic Arabidopsis overexpressing ScPIP1.
Highlights
Aquaporins transport water, CO2 and small neutral solutes through the plasma and intracellular membranes of cells [1,2,3]
The results of malonaldehyde (MDA), ion leakage (IL) and proline content measurements indicated that the improved drought and salt tolerance conferred by ScPIP1 was correlated with decreased membrane damage and improved osmotic adjustment
We found that the EST sequence (Genbank accession number: DV752738) of aquaporin was highly repeated, indicating that this gene was regulated by water deficit [30]
Summary
Aquaporins transport water, CO2 and small neutral solutes through the plasma and intracellular membranes of cells [1,2,3]. They play a central role in regulating water transport in many physiological and developmental processes, including cell elongation, stomatal regulation, seed germination, reproductive growth and stress responses in plants [4,5]. The symplastic pathway in which aquaporins (AQPs) play a central role is efficient for transporting water across membranes [11,12,13,14]
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