Abstract
Talinum paniculatum plants contain flavonoids and saponins, which have strong antioxidant properties and are used in the treatment of numerous diseases, such as inflammation, allergies, and gastric ulcers. Currently, there is no published research on the flavonoid content of T. paniculatum plants; however, it has been determined that the species in the genus Talinum have very low flavonoid content, and therefore the effective approach to enhance the flavonoid content in T. paniculatum plants is overexpression of the Glycine max chalcone isomerase (GmCHI) gene. In this study, we analyzed GmCHI gene overexpression, which has increased total flavonoid content in transgenic T. paniculatum lines. We have successfully transformed T. paniculatum plants with the pCB301-GmCHI vector and created 2 lines in generation T1 (T1-2.2 and T1-10) with a transgenic frequency of 0.27%. Recombinant CHI protein expressed in transgenic lines has a molecular weight of approximately 25 kDa, and the concentration of this protein in the two transgenic lines T1-2.2 and T1-10 was 4.29 ?g mg?1 and 6.14 ?g mg?1, respectively. Total flavonoid contents of the two transgenic lines T1-2.2 and T1-10 were 4.24 mg g?1 and 2.74 mg g?1, respectively, which showed increases of 7.4-fold and 4.8-fold compared to that of the nontransgenic plants, respectively. Thus, overexpression of the GmCHI gene enabled T. paniculatum plants to improve their total flavonoid content. This work also confirmed the effectiveness of Agrobacterium-mediated plant transformation in enhancement of flavonoid accumulation in T. paniculatum plants.
Highlights
Flavonoids are groups of secondary metabolites that play a variety of significant roles in plants
We present the results of overexpressing the Glycine max chalcone isomerase (GmCHI) gene, which was isolated from soybean, in T. paniculatum plants to increase flavonoid content in transgenic T. paniculatum lines
Cotyledons were infected with A. tumefaciens carrying the pCB301-Chalcone isomerase (CHI) transformation vector
Summary
Flavonoids are groups of secondary metabolites that play a variety of significant roles in plants. Flavonoids play important roles in human health They are natural sources of antioxidants, which control many different human diseases (Kim et al, 2003). Several flavonoids such as catechin, apigenin, quercetin, naringenin, rutin, and venoruton are reported to have hepatoprotective activities (Tapas et al, 2008) that prevent the occurrence of age-related chronic diseases, such as cardiovascular disease or some types of cancer (Butelli et al, 2008; Luceri et al, 2008). Flavonoids are synthesized by the phenylpropanoid biosynthesis pathway, a fundamental secondary synthesis pathway in all higher
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
