Abstract
The product of the START gene CDC25, an upstream element of the RAS/adenylyl cyclase pathway in Saccharomyces cerevisiae, was identified using specific antibodies raised against a chimeric β-galactosidase/CDC25 protein. The CDC25 protein is poorly expressed and can be detected only when the CDC25 gene is overexpressed under the control of the galactose-inducible GAL1–10 strong promoter elements. It has a molecular weight of 180, 000, is not glycosylated and is strongly associated with the particulate fraction. After deletion of residues 1255–1550 the protein is found in the soluble fraction.
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