Abstract
BackgroundRosea1 (Ros1) and Delila (Del) co-expression controls anthocyanin accumulation in snapdragon flowers, while their overexpression in tomato strongly induces anthocyanin accumulation. However, little data exist on how Del expression alone influences anthocyanin accumulation.ResultsIn tobacco (Nicotiana tabacum ‘Xanthi’), Del expression enhanced leaf and flower anthocyanin production through regulating NtCHS, NtCHI, NtF3H, NtDFR, and NtANS transcript levels. Transgenic lines displayed different anthocyanin colors (e.g., pale red: T0-P, red: T0-R, and strong red: T0-S), resulting from varying levels of biosynthetic gene transcripts. Under salt stress, the T2 generation had higher total polyphenol content, radical (DPPH, ABTS) scavenging activities, antioxidant-related gene expression, as well as overall greater salt and drought tolerance than wild type (WT).ConclusionWe propose that Del overexpression elevates transcript levels of anthocyanin biosynthetic and antioxidant-related genes, leading to enhanced anthocyanin production and antioxidant activity. The resultant increase of anthocyanin and antioxidant activity improves abiotic stress tolerance.
Highlights
Rosea1 (Ros1) and Delila (Del) co-expression controls anthocyanin accumulation in snapdragon flowers, while their overexpression in tomato strongly induces anthocyanin accumulation
The T0 transgenic phenotypes displayed stem and leaf colors corresponding to differing anthocyanin accumulation levels: pale red (T0-P), red (T0-R), and strong red (T0-S) (Fig. 1a)
Upon transferal to a greenhouse alongside wild type (WT), anthocyanin accumulation was maintained in transgenic lines and obvious between-line phenotypic variation was observed (Fig. 1b)
Summary
Rosea (Ros1) and Delila (Del) co-expression controls anthocyanin accumulation in snapdragon flowers, while their overexpression in tomato strongly induces anthocyanin accumulation. Results: In tobacco (Nicotiana tabacum ‘Xanthi’), Del expression enhanced leaf and flower anthocyanin production through regulating NtCHS, NtCHI, NtF3H, NtDFR, and NtANS transcript levels. Transgenic lines displayed different anthocyanin colors (e.g., pale red: T0-P, red: T0-R, and strong red: T0-S), resulting from varying levels of biosynthetic gene transcripts. Anthocyanins are plant pigments produced via a dedicated biosynthetic pathway in flowers, fruit, leaves, and stems. Anthocyanin production was enhanced in tomato with the co-expression of snapdragon (Antirrhinum majus)-derived bHLH transcription factor Delila (Del) and the MYB transcription factor Rosea (Ros1) [2, 3]. AtMYB12, Del, and Ros overexpression enhanced anthocyanin biosynthetic gene expression, as well as activating primary metabolism [4]. More data are necessary to fully understand of the involvement of this gene in anthocyanin-linked flower pigmentation
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