Overexpression of SlGRAS7 Affects Multiple Behaviors Leading to Confer Abiotic Stresses Tolerance and Impacts Gibberellin and Auxin Signaling in Tomato.

Sidra Habib,Ning Li,Muhammad Waseem,Lu Yang,Zhengguo Li

doi:10.1155/2019/4051981

Abstract

Abiotic stresses remain the key environmental issues that reduce plant development and therefore affect crop production. Transcription factors, such as the GRAS family, are involved in various functions of abiotic stresses and plant growth. The GRAS family of tomato (Solanum lycopersicum), SlGRAS7, is described in this study. We produced overexpressing SlGARS7 plants to learn more about the GRAS transcription factors. Plants overexpressing SlGARS7 (SlGRAS7-OE) showed multiple phenotypes related to many behaviors, including plant height, root and shoot length, and flowering time. We observed that many genes in the SlGRAS7-OE seedlings that are associated with auxin and gibberellin (GA) are downregulated and have altered sensitivity to GA3/IAA. SlGRAS7 was upregulated during abiotic stresses following treatment with sodium chloride (NaCl) and D-mannitol in the wild-type (WT) tomato. Tomato plants overexpressing SlGRAS7 showed more resistance to drought and salt stress comparison with WT. Our study of SlGRAS7 in tomato demonstrates how GRAS showed an integrative role, improving resistance to abiotic stresses and enhancing gibberellin/auxin signaling through reproductive as well as vegetative processes.

Highlights

The relationship between molecular developments and environmental clues is measured by the important modifications in gene regulatory networks (GRNs), which play a dynamic part in the manipulability of development and growth in plants [1,2,3]
GRAS proteins are categorized into 13 subfamilies, containing HAM, AtSCR, AtSCL3, AtSCL4/7, AtSCL9, AtSCL28, AtSHR, AtLAS, AtPAT1, Os4, Os19, DELLA, and Pt20 in Arabidopsis, rice, and Populus according to the phylogenetic tree and amino acid sequence alignment [15]
A GRAS transcription factor obtained from Vitis amurensis was used to create transgenic Arabidopsis, and the overexpression of VaPAT1 leads to drought tolerance, high salinity, and cold stress [20]

Summary

Introduction

The relationship between molecular developments and environmental clues is measured by the important modifications in gene regulatory networks (GRNs), which play a dynamic part in the manipulability of development and growth in plants [1,2,3]. The GRAS gene family (named after GAI, RGA, and SCR) is induced through various abiotic stresses [6, 7] Transcription factors, such as the GRAS proteins, which are involved in plant growth and pathways of signal transduction, are involved in lateral shoot development [8], phytochrome signaling [9], gametogenesis [10], auxin signaling [11, 12], and gibberellin signaling and biosynthesis [13, 14]. By inhibition of miR156-targeted SPL proteins, the miR171-GRAS component controls trichome distribution and flowering time [26] This component is critical for stimulating GA-DELLA signaling in the organization of leaf development in the light and regulation of chlorophyll biosynthesis [27]. Many studies showed that GRAS proteins play many significant functions in the tolerance of abiotic or biotic stress

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