Abstract
BackgroundSuppressor anaphase-promoting complex domain containing 2 (SAPCD2) is a novel gene playing important roles in the initiation, invasion, and metastasis of several malignancies. However, its role in colorectal carcinoma (CRC) still remains unclear.MethodIn this study, we investigated the expression and biological function of SAPCD2 in CRC. Immunohistochemistry (IHC) for SAPCD2 was performed in 410 pairs of CRC specimens and corresponding normal epithelial tissues, and in 50 adenoma tissues. Clinical pathological factors were analyzed in relation to the expression of SAPCD2. The biological functions of SAPCD2 in CRC cells and its effect on cell cycle were investigated in vitro and in vivo through gain/loss-of-function approaches.ResultsIHC showed that SAPCD2 expression was significantly higher in CRC tissues compared to adenoma and normal epithelium tissues and was correlated with tumor location (p = 0.018). SAPCD2 significantly promoted cell proliferation, migration, and invasion both in vitro and in vivo (p < 0.05). In addition, SAPCD2 knockdown in CRC cells was associated with reduced G1/S transition, while overexpression caused G2/M phase arrest (p < 0.05).ConclusionsIn sum, SAPCD2 is overexpressed in CRC tissues and plays a critical role in CRC progression. Therefore, it might represent a promising therapeutic target for CRC treatment.
Highlights
Suppressor anaphase-promoting complex domain containing 2 (SAPCD2) is a novel gene playing important roles in the initiation, invasion, and metastasis of several malignancies
IHC showed that SAPCD2 expression was significantly higher in CRC tissues compared to adenoma and normal epithelium tissues and was correlated with tumor location (p = 0.018)
SAPCD2 expression is elevated in CRC tissues IHC staining was performed to analyze the expression of SAPCD2 in CRC, adenoma, and normal epithelium tissues from 410 CRC and 50 adenoma patients
Summary
Suppressor anaphase-promoting complex domain containing 2 (SAPCD2) is a novel gene playing important roles in the initiation, invasion, and metastasis of several malignancies. Suppressor anaphase-promoting complex domain containing 2 (SAPCD2), known as p42.3 or C9orf140, is Recently, several studies have reported that SAPCD2 is overexpressed in several kinds of solid tumors, including GC, hepatocellular carcinoma (HCC), melanoma, glioblastoma, and renal cell cancer (RCC) [2, 6,7,8,9], and might play important roles in cell proliferation, migration, and invasion by activating JAK/STAT, MAPK, and Wnt signaling pathways [3, 4, 7, 9]. Little is known about the function of SAPCD2 in colorectal carcinoma (CRC)
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