Overexpression of S-Adenosyl-l-Methionine Synthetase 2 from Sugar Beet M14 Increased Arabidopsis Tolerance to Salt and Oxidative Stress.

Chunquan Ma,Dan Gu,Yuguang Wang,Jingdong Nan,Sixue Chen,Haiying Li

doi:10.3390/ijms18040847

Chunquan Ma, Dan Gu + Show 4 more

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https://doi.org/10.3390/ijms18040847

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Abstract

The sugar beet monosomic addition line M14 is a unique germplasm that contains genetic materials from Beta vulgaris L. and Beta corolliflora Zoss, and shows tolerance to salt stress. Our study focuses on exploring the molecular mechanism of the salt tolerance of the sugar beet M14. In order to identify differentially expressed genes in M14 under salt stress, a subtractive cDNA library was generated by suppression subtractive hybridization (SSH). A total of 36 unique sequences were identified in the library and their putative functions were analyzed. One of the genes, S-adenosylmethionine synthetase (SAMS), is the key enzyme involved in the biosynthesis of S-adenosylmethionine (SAM), a precursor of polyamines. To determine the potential role of SAMS in salt tolerance, we isolated BvM14-SAMS2 from the salt-tolerant sugar beet M14. The expression of BvM14-SAMS2 in leaves and roots was greatly induced by salt stress. Overexpression of BvM14-SAMS2 in Arabidopsis resulted in enhanced salt and H2O2 tolerance. Furthermore, we obtained a knock-down T-DNA insertion mutant of AtSAMS3, which shares the highest homology with BvM14-SAMS2. Interestingly, the mutant atsam3 showed sensitivity to salt and H2O2 stress. We also found that the antioxidant system and polyamine metabolism play an important role in salt and H2O2 tolerance in the BvM14-SAMS2-overexpressed plants. To our knowledge, the function of the sugar beet SAMS has not been reported before. Our results have provided new insights into SAMS functions in sugar beet.

Highlights

Soil salinity is a serious ecological problem that affects crop distribution and yield around the world [1,2,3]
The results demonstrated that the overexpression of BvM14-SAMS2 can confer salt and H2O2 stress tolerance in the transgenic plants
The cDNAs synthesized from the sugar beet M14 root and leaf mRNAs under control conditions were used as drivers, and those from roots and leaves under salt stress were selected as testers

Summary

Introduction

Soil salinity is a serious ecological problem that affects crop distribution and yield around the world [1,2,3]. Improving the salt tolerance of crops to utilize saline soil is of high urgency [5,6]. In order to adapt to the saline environment, plants can use some strategies allowing for adaptation, which include efflux of salt ions, compartmentalization of Na+ in vacuoles, synthesis of osmolytes, and increased synthesis of antioxidant enzymes [7]. In these adaptation processes, salt stress regulatory genes are induced, leading to changes in the protein levels that enable adaptation to the salinity conditions. Numerous proteins have exhibited salt stress responses as identified by proteomics studies in several halophytes [12,13,14,15]

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