Abstract
SK-N-SH human neuroblastoma cell line was employed to test whether direct introduction of the RII beta subunit of PKA could induce growth inhibition and reverse-transformation of cancer cells. We infected SK-N-SH cells with an RII beta expression construct and the clonal line with the highest expression level was selected to examine the effect of RII beta overexpression. Elevation of RII beta was accompanied by marked reduction of RI alpha level and increase of C alpha level. Since the mRNA levels of RI alpha and C alpha were not altered by increased RII beta, the changes in the level of RI alpha and C alpha protein seem to be due to a post-transcriptional event. The RII beta-overexpressing cell line, SK-RII beta, exhibited retarded monolayer growth, decreased DNA synthesis, inability to grow in soft agar, and reduced viability in serum-free or low-serum condition as compared to parental cell, SK-N-SH. These characteristics of SK-RII beta disappeared when the RII beta expression was not fully induced. These results showed that the elevation of RII beta and/or the decrease of RI alpha is closely related to the suppression of cell proliferation and the status of transformation of SK-N-SH neuroblastoma cell line.
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