Overexpression of Periostin and Lumican in Esophageal Squamous Cell Carcinoma

Manoj Kumar Kashyap,Elizabeth A Montgomery,K V Veerendra Kumar,Suraj Peri,Akhilesh Pandey,Rekha V Kumar,Thottethodi Subrahmanya Keshava Prasad,Harrys K.C Jacob,M Vijaya Kumar,Riaz Mahmood,Ghantasala S Sameer Kumar,Stephen J Meltzer,Arivusudar Marimuthu

doi:10.3390/cancers2010133

Abstract

To identify biomarkers for early detection for esophageal squamous cell carcinoma (ESCC), we previously carried out a genome-wide gene expression profiling study using an oligonucleotide microarray platform. This analysis led to identification of several transcripts that were significantly upregulated in ESCC compared to the adjacent normal epithelium. In the current study, we performed immunohistochemical analyses of protein products for two candidates genes identified from the DNA microarray analysis, periostin (POSTN) and lumican (LUM), using tissue microarrays. Increased expression of both periostin and lumican was observed in 100% of 137 different ESCC samples arrayed on tissue microarrays. Increased expression of periostin and lumican was observed in carcinoma as well as in stromal cell in the large majority of cases. These findings suggest that these candidates can be investigated in the sera of ESCC patients using ELISA or multiple reaction monitoring (MRM) type assays to further explore their utility as biomarkers.

Highlights

IntroductionWe previously reported identification of a number of genes significantly upregulated in esophageal squamous cell carcinoma (ESCC) using DNA microarrays [2]
esophageal squamous cell carcinoma (ESCC) is the predominant type of esophageal cancer in the developing world [1]
Extracellular matrix proteins are increasingly being demonstrated to be involved in the process of epithelial-to-mesenchymal transition events leading to tumor progression

Summary

Introduction

We previously reported identification of a number of genes significantly upregulated in ESCC using DNA microarrays [2]. Among these were several differentially expressed ECM genes such as matrix metalloproteinase 13 (MMP13), hyaluronic acid synthase 3 (HAS3), aggrecan 1 chondroitin sulfate proteoglycan 1 (AGC1), secreted protein acidic cysteine rich osteonectin (SPARC), matrix metalloproteinase 11 (MMP11), serine or cysteine proteinase inhibitor clade b ovalbumin member 11. Components of the extracellular matrix (ECM) are key molecules in tumor-stroma interactions, which are believed to be involved in progression of different tumor types. Periostin is a secreted extracellular matrix protein that belongs to the fasciclin gene family

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Results

Conclusion