Abstract
Anthocyanins, a categories of metabolites derived from flavonoid biosynthesis pathway, are responsible for the red color of peach. Anthocyanin metabolism is modulated by a complicated regulatory network. In this study, our results demonstrated that PpNAC25 was a component of anthocyanin regulatory network in peach. <italic>PpNAC25</italic> showed a closed relationship with the well-known ripening-related gene <italic>NOR</italic> (<italic>no-ripening</italic>) and was highly expressed in peach fruit. Overexpression of <italic>PpNAC25</italic> in poplar resulted in a redder shoot tips compared with EV control. <italic>PpNAC25</italic> overexpression upregulated the anthocyanin biosynthetic and transportation genes in transcript levels and then increased anthocyanin contents. In Y1H and Luc/Ren assay, PpNAC25 bound to the promoter of the anthocyanin-activator <italic>PpMYB10.1</italic> and <italic>PpMYB10.2</italic> and activated its transcript. Moreover, PpNAC25 formed a heterodimer with PpNAC1, an anthocyanin-regulator. Our researches provide evidence that PpNAC25 may be a positive regulator of anthocyanin biosynthesis in peach fruit.
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