Overexpression of OsHSP18.0-CI Enhances Resistance to Bacterial Leaf Streak in Rice

Yanhu Ju,Zhaohui Chu,Liping Zuo,Guixiu Jin,Xiangkui Li,Xinhua Ding,Ruihua Zhang,Qian Xu,Hongjuan Tian

doi:10.1186/s12284-017-0153-6

Yanhu Ju, Zhaohui Chu + Show 7 more

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https://doi.org/10.1186/s12284-017-0153-6

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Abstract

BackgroundThe small heat shock proteins represent a large family of proteins that respond to a wide range of abiotic and biotic stresses. OsHsp18.0-CI confers tolerance to salt and cadmium and interacts with viral RNA-dependent RNA polymerase (RdRp). However, the direct function of OsHsp18.0-CI in resistance against biotic stresses remains unclear in rice.ResultsHere, we report that the expression of OsHsp18.0-CI was up-regulated upon inoculation with RS105, a strain of Xanthomonas oryzae pv. oryzicola (Xoc) that causes bacterial leaf streak in rice. In comparison with wild-type, OsHsp18.0-CI overexpression (OE) lines exhibited enhanced resistance to RS105, whereas repression lines exhibited compromised resistance to RS105. In addition, the transcriptional profiles of wild type and OE lines were compared with and without inoculation with RS105. After inoculation with RS105, most of the genes with up-regulated expression were commonly stimulated in the wild type and OE lines, with stronger induction in the OE lines than in wild type.ConclusionOur study reveals that OsHsp18.0-CI positively regulates resistance to Xoc by mediating an enhanced version of the basal defense response in rice.

Highlights

The small heat shock proteins represent a large family of proteins that respond to a wide range of abiotic and biotic stresses
The Expression of OsHsp18.0-CI is Induced by Xanthomonas oryzae pv. oryzicola (Xoc) As the expression of OsHsp18.0-CI is activated by M. grisea and rice viruses (Sarkar et al 2009; Li et al 2015), we determined if it is induced by Xoc
We measured the expression of OsHsp18.0-CI postinoculation with the Xoc strain RS105 in the susceptible rice variety Shengdao 806

Summary

Introduction

The small heat shock proteins represent a large family of proteins that respond to a wide range of abiotic and biotic stresses. It was identified that qBlsr5a is mainly controlled by xa, a major gene for resistance to bacterial blight caused by Xanthomonas oryzae pv. A new dominant resistance locus named Xo1 has been identified in the qualitative resistance against the African clade of Xoc strains of the American heirloom rice variety Carolina Gold Select (Triplett et al 2016). This resistance can be triggered by a transcription activator-like (TAL) effector in Xoc (Triplett et al 2016). A non-host resistance gene, Rxo, which encodes a typical plant resistance protein containing a nucleotide-binding site-leucine rich repeat (NBS-LRR)

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