Abstract
Dof (DNA binding with one finger) proteins, a class of plant-specific transcription factors, are involved in plant growth and developmental processes and stress responses. However, their biological functions remain to be elucidated, especially in rice (Oryza sativa L.). Previously, we have reported that OsDof12 can promote rice flowering under long-day conditions. Here, we further investigated the other important agronomical traits of the transgenic plants overexpressing OsDof12 and found that overexpressing OsDof12 could lead to reduced plant height, erected leaf, shortened leaf blade, and smaller panicle resulted from decreased primary and secondary branches number. These results implied that OsDof12 is involved in rice plant architecture formation. Furthermore, we performed a series of Brassinosteroid (BR)-responsive tests and found that overexpression of OsDof12 could also result in BR hyposensitivity. Of note, in WT plants the expression of OsDof12 was found up-regulated by BR treatment while in OsDof12 overexpression plants two positive BR signaling regulators, OsBRI1 and OsBZR1, were significantly down-regulated, indicating that OsDof12 may act as a negative BR regulator in rice. Taken together, our results suggested that overexpression of OsDof12 could lead to altered plant architecture by suppressing BR signaling. Thus, OsDof12 might be used as a new potential genetic regulator for future rice molecular breeding.
Highlights
DOF (DNA binding with one finger) proteins are plant-specific transcription factors (Yanagisawa, 2002; Moreno-Risueno et al, 2007; Noguero et al, 2013)
We focused on overexpression of OsDof12 promoting flowering in rice under long-day conditions
We demonstrate that overexpression of OsDof12 can alter the rice plant architecture
Summary
DOF (DNA binding with one finger) proteins are plant-specific transcription factors (Yanagisawa, 2002; Moreno-Risueno et al, 2007; Noguero et al, 2013). With the exception in pumpkin, usually regulate the expression of the target genes via binding a core DNA motif with an essential sequence of AAAG (Yanagisawa and Schmidt, 1999). Dof proteins are widespread and versatile regulators for various biological processes such as metabolism regulation, phytohormone response, seed germination and development, photoperiodic flowering and plant patterning in plants (Yanagisawa, 2002; Noguero et al, 2013). Several cases have documented that Dof genes are implicated in seed germination and development. RPBF (rice prolamin box binding factor) interplayed with the rice basic leucine zipper factor RISBZ1 to maintain proper expression of rice seed storage protein genes during seed development (Kawakatsu et al, 2009)
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