Abstract
Rice stripe virus (RSV) causes a severe disease in Oryza sativa (rice) in many Eastern Asian countries. The NS3 protein of RSV is a viral suppressor of RNA silencing, but plant host factors interacting with NS3 have not been reported yet. Here, we present evidence that expression of RSV NS3 in Arabidopsis thaliana causes developmental abnormalities. Through yeast two-hybrid screening and a luciferase complementation imaging assay, we demonstrate that RSV NS3 interacted with OsCIPK30, a CBL (calcineurin B-like proteins)-interaction protein kinase protein. Furthermore, OsCIPK30 was overexpressed to investigate the function of OsCIPK30 in rice. Our investigation showed that overexpression of OsCIPK30 in rice could delay the RSV symptoms and show milder RSV symptoms. In addition, the expression of pathogenesis-related genes was increased in OsCIPK30 transgenic rice. These results suggest that overexpression of OsCIPK30 positively regulates pathogenesis-related genes to enhance the tolerance to RSV in rice. Our findings provide new insight into the molecular mechanism underlying resistance to RSV disease.
Highlights
Rice stripe disease, caused by Rice stripe virus (RSV), is one of the most destructive diseases in rice (Wei et al, 2009)
The phenotypes of transgenic plants indicated that NS3 might impact growth and development in Arabidopsis and might influence rice
To investigate whether RSV can induce the expression of OsCIPKs (OsCIPK5, OsCIPK25, OsCIPK26, OsCIPK29, OsCIPK30, and OsCIPK32), we examined the relative transcript level of these six OsCIPK genes in RSV-infected rice at 7 days post-inoculation
Summary
Rice stripe disease, caused by Rice stripe virus (RSV), is one of the most destructive diseases in rice (Wei et al, 2009). RSV was first reported in Japan in 1897 (Kisimoto, 1965) and is currently present in many East Asian countries, including China, where it was first reported in 1963. It is widely spread in at least 16 different provinces of China (Wei et al, 2009). Rice stripe virus belongs to the Tenuivirus genus, and the viral genome consists of four singlestranded RNA segments (Hibino, 1996; Falk and Tsai, 1998), which encode seven ORFs using a negative or ambisense coding strategy (Ramirez and Haenni, 1994). RSV vRNA2 encodes a membrane-associated protein ( named NS2)
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