Abstract
Gastric cancer is one of the most common cancers worldwide. The purpose of this study was to find out potential markers for gastric cancer. Tumor and normal tissues from 152 gastric cancer cases were analyzed by two-dimensional gel electrophoresis (2-DE). The images of silver stained gels were analyzed and statistical analysis of spot intensities revealed that spot 4262 showed higher expression (5.7-fold increase) in cancer tissues than in normal tissues (P < 0.001). It was identified by peptide mass fingerprinting as nicotinamide N-methyltransferase (NNMT). A monoclonal antibody with a detection limit down to 10 ng was produced against NNMT in mouse. Using the prepared monoclonal antibody, western blot analysis of NNMT was performed for gastric tissues from 15 gastric cancer patients and two gastric ulcer patients. The results corroborated those of 2-DE experiments. A single spot was detected in gastric ulcer tissues while four to five spots were detected in gastric cancer tissues. In cancer tissues, two additional spots of acidic and basic form were mainly detected on 2-DE gels. This suggests that NNMT receives a post-translational modification in cancer- specific manner.
Highlights
Gastric cancer is the most common cancer in Korea and other Asian countries
Proteomics is one of the technologies that rapidly change our approach to cancer research and can lead to the molecular characterization of cellular events associated with cancer progression, signaling, and developmental stages. 152 cases of gastric cancer and normal tissues were analyzed by two-dimensional electrophoresis, and western blotting
Cases of gastric cancer and normal tissues were analyzed by two-dimensional electrophoresis
Summary
Gastric cancer is the most common cancer in Korea and other Asian countries. incidence rates in western world are much lower than in Asia, gastric carcinoma is still a significant worldwide health burden, second only to lung tumors as a leading cause of cancer deaths (Lyman, 1992; Ha et al, 2002; Kim et al, 2002). Two-dimensional gel electrophoresis (2-DE) is widely used for in investigation of protein complements between diseased and healthy tissue with the purpose of developing diagnostic markers and detecting novel drug targets (Wilkins et al, 1996; Gygi et al, 2000). Proteomic technologies are providing the tools needed to discover and identify biomarkers associated with diverse diseases and biological processes (Blackstock et al, 1999; Hanash, 2003; Choi et al, 2004; Eun et al, 2004). Proteomics is one of the technologies that rapidly change our approach to cancer research and can lead to the molecular characterization of cellular events associated with cancer progression, signaling, and developmental stages. As a result of this study, we suggest that nicotinamide N-methyltransferase will be an efficient tumor marker in gastric cancer diagnosis
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