Abstract
Potential of MoSM1, encoding for a cerato-platanin protein from Magnaporthe oryzae, in improvement of rice disease resistance was examined. Transient expression of MoSM1 in rice leaves initiated hypersensitive response and upregulated expression of defense genes. When transiently expressed in tobacco leaves, MoSM1 targeted to plasma membrane. The MoSM1-overexpressing (MoSM1-OE) transgenic rice lines showed an improved resistance, as revealed by the reduced disease severity and decreased in planta pathogen growth, against 2 strains belonging to two different races of M. oryzae, causing blast disease, and against 2 strains of Xanthomonas oryzae pv. oryzae, causing bacterial leaf blight disease. However, no alteration in resistance to sheath blight disease was observed in MoSM1-OE lines. The MoSM1-OE plants contained elevated levels of salicylic acid (SA) and jasmonic acid (JA) and constitutively activated the expression of SA and JA signaling-related regulatory and defense genes. Furthermore, the MoSM1-OE plants had no effect on drought and salt stress tolerance and on grain yield. We conclude that MoSM1 confers a broad-spectrum resistance against different pathogens through modulating SA- and JA-mediated signaling pathways without any penalty on abiotic stress tolerance and grain yield, providing a promising potential for application of MoSM1 in improvement of disease resistance in crops.
Highlights
Before the investigation of function of MoSM1 in rice defense, we further examined the biochemical features of MoSM1 such as the role of signal peptide (SP) in activity and subcellular localization in planta
When transiently expressed in leaves of N. benthamiana plants, significant accumulation of eGFP-MoSM1 fusion was detected by Western blotting, which was similar to the accumulation of eGFP alone, at 24 hr after agroinfiltration (Fig. 1b)
Microscopic observation revealed that the eGFP-MoSM1 fusion targeted mainly to the plasma membrane, co-localized with a previously reported plasma membrane-targeted Arabidopsis aquaporin AtPIP1;4, whereas the eGFP alone distributed throughout the cells with distribution in nucleus, at 24 hr after agroinfiltration (Fig. 1c)
Summary
SM1 and SM2 from Trichoderma virens, BcSpl[1] from B. cinerea, and MoSM1 ( known as MoMSP1) from M. oryzae could induce systemic disease resistance in rice, cotton, maize, tobacco, tomato and Arabidopsis against different pathogens[32,37,38,40,42,45,46,47] and the activation of systemic resistance by CP from C. platani and BcSpl[1] from B. cinerea was found to be regulated through stomatal perception, overexpression of salicylic acid (SA)- and ethylene-signaling genes and camalexin biosynthesis[44,46]. The MoSM1-OE plants did not affect the drought and salt stress tolerance and some of the important agronomic traits such as grain yield
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