Overexpression of miR-758 inhibited proliferation, migration, invasion, and promoted apoptosis of non-small cell lung cancer cells by negatively regulating HMGB.

Guo-Hua Zhou,Xiao-Bo Wu,Yi-Yu Lu,Zi-Kun Gao,Wei-Guang Gu,Jing-Lian Xie,Wei-Shen Yao

doi:10.1042/bsr20180855

Guo-Hua Zhou, Xiao-Bo Wu + Show 5 more

Open Access

https://doi.org/10.1042/bsr20180855

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Abstract

Non-small cell lung cancer (NSCLC) is one of the most fatal types of cancer with significant mortality and morbidity worldwide. MicroRNAs (miRs) have been confirmed to have positive functions in NSCLC. In the present study, we try to explore the role of miR-758 in proliferation, migration, invasion, and apoptosis of NSCLC cells by regulating high-mobility group box (HMGB) 3 (HMGB3.) NSCLC and adjacent tissues were collected. Reverse transcription quantitative PCR (RT-qPCR) was employed to detect expression of miR-758 and HMGB3 in NSCLC and adjacent tissues, in BEAS-2B cells and NSCLC cell lines. The targetted relationship between miR-758 and HMGB3 was identified by dual luciferase reporter gene assay. The effects of miR-758 on proliferation, migration, invasion, cell cycle, and apoptosis of A549 cells. MiR-758 expression was lower in NSCLC tissues, which was opposite to HMGB3 expression. The results also demonstrated that miR-758 can target HMGB3. The cells transfected with miR-758 mimic had decreased HMGB3 expression, proliferation, migration, and invasion, with more arrested cells in G1 phase and increased apoptosis. Our results supported that the overexpression of miR-758 inhibits proliferation, migration, and invasion, and promotes apoptosis of NSCLC cells by negative regulating HMGB2. The present study may provide a novel target for NSCLC treatment.

Highlights

Non-small cell lung cancer (NSCLC) is a prevalent form of lung cancer in the elderly, and its incidence is predicted to substantially increase [1]
The miR-758 expression in NSCLC and adjacent tissues was determined by reverse transcription quantitative PCR (RT-qPCR), showing that amongst the 50 pairs of NSCLC tissues, the miR-758 expression in 36 pairs of tissues presented with a lower miR-758 expression than adjacent tissues, with 14 pairs with significantly lower than that the adjacent tissues (>two-fold decrease)
The HMGB3 expression in NSCLC and adjacent tissues was measured by Reverse transcription quantitative PCR (RT-qPCR), showing that amongst the 50 cases of NSCLC tissues, the mRNA level of HMGB3 in 34 cases of tissues presented with a higher expression than the adjacent tissues, along with 16 cases of significantly higher (>two-fold increase) than the adjacent tissues (P

Summary

Introduction

Non-small cell lung cancer (NSCLC) is a prevalent form of lung cancer in the elderly, and its incidence is predicted to substantially increase [1]. Before 2009, NSCLC was treated by cytotoxic chemotherapy which provided a 20–35% response rate and a 10–12 months median survival time [6]. The NSCLC patients treated with epidermal growth factor receptor inhibitors have an increased median survival time up to 18 months [7]. Resistance to current chemotherapy for the treatment of NSCLC is one of the main obstacles to improve long-term efficacy for patients [9]. Surgical resection is the most promising approach to cure NSCLC with c 2019 The Author(s).

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