Overexpression of miR-140 in epithelial ovarian cancer cell lines treated with 5-Aza-2′-deoxycytidine

Abstract

Cancer is a growing global problem, and ovarian cancer is one of the deadliest cancers in women. Today, in addition to pharmacological treatment of cancer, efforts are being made to explore new ways, including microRNAs, as one of the new biomarkers for the prognosis, diagnosis and treatment of this cancer.In this study, the effect of cisplatin and epigenetic chemotherapy drug 5-Aza-2′-deoxycytidine, which inhibits DNA methyl transferase enzymes on cancer cell lines sensitive and resistant to chemotherapy of A2780s and A2780cp ovarian cancer cell lines.The results showed that DNMT1 expression was increased and miR-140 expression was decreased in cancer cell lines. Using cisplatin and 5-Aza-2′-deoxycytidine separately and in combination, DNMT1 expression decreased and miR-140 expression increased. This result could suggest this microRNA as a suitable biomarker and 5-Aza-2′-deoxycytidine as an effective drug for cancer treatment. Due to the more important effects of 5-Aza-2′-deoxycytidine in combination with cisplatin than the effects of monotherapy, this combination therapy is recommended for the treatment of ovarian cancer, especially those resistant to chemotherapy.