Abstract
BackgroundCompelling evidences reported the role of microRNAs (miRNAs) in ovarian cancer. However, little was known regarding the molecular mechanism of miR-367 in ovarian cancer. This study intended to investigate the role and regulatory mechanism of miR-367 in ovarian cancer involving lysophosphatidic acid receptor-1 (LPA1).MethodsPotentially regulatory miRNAs in ovarian cancer were obtained from bioinformatics analysis. RT-qPCR was used to detect miR-367 expression in both ovarian cancer tissues and relevant adjacent normal tissues. Relationship between miR-367 and LPA1 was predicted by miRNA database and further verified using dual luciferase reporter gene assay and RIP. EdU and Transwell assay were used to measure the proliferation and invasion ability of cells. Moreover, tube formation and chick chorioallantois membrane (CAM) assay were performed to determine angiogenesis of human umbilical vein endothelial cells (HUVECs). Finally, the roles of LPA1 in tumor growth was also studied using nude mice xenograft assay.ResultsHigh expression of LPA1 and low expression of miR-367 were observed in ovarian cancer tissues and cells. Overexpressed miR-367 downregulated LPA1 expression to inhibit proliferation, invasion, and angiogenesis of cancer cells. Low expression of LPA1 suppressed tumor formation and repressed angiogenesis in ovarian in vivo.ConclusionAll in all, overexpression of miR-367 downregulated LPA1 expression to inhibit ovarian cancer progression, which provided a target for the cancer treatment.
Highlights
Compelling evidences reported the role of microRNAs in ovarian cancer
Results miR‐367 might participate in ovarian cancer progression by regulating lysophosphatidic acid receptor-1 (LPA1) To screen out the miRNA involved in ovarian cancer, the miRNA expression microarray dataset GSE48485 and mRNA expression microarray dataset GSE66957 of ovarian cancer were obtained from the Gene Expression Omnibus (GEO) database
LPA1 was found to be highly expressed in cancer metastatic cell lines, and LPA1 overexpression could promote the invasion and migration of ovarian cancer cells [18]
Summary
Compelling evidences reported the role of microRNAs (miRNAs) in ovarian cancer. Little was known regarding the molecular mechanism of miR-367 in ovarian cancer. This study intended to investigate the role and regulatory mechanism of miR-367 in ovarian cancer involving lysophosphatidic acid receptor-1 (LPA1). The progression of ovarian cancer requires the co-evolution of neoplastic cells as well as the adjacent microenvironment [2]. It was reported that the main challenge of ovarian cancer treatment was that most patients have advanced disease at the time of diagnosis [3]. MicroRNAs (miRNAs) are a variety of RNAs that could regulate the translation and stability of mRNAs influencing cell differentiation, migration and apoptosis [7]. It has been verified that miRNAs are differentially expressed in ovarian cancer and exert functions as
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