Abstract

BackgroundLung cancer is the leading cause of cancer-related deaths worldwide. Early detection is considered critical for lung cancer treatment. MicroRNAs (miRNAs) have shown promise as diagnostic and prognostic indicators. This study was to identify specific miRNAs with diagnostic and prognostic value for patients with lung cancer, and to explore the correlation between expression profiles of miRNAs and patient survival.MethodsGene expression of members of the miR-183 family (miR-96, miR-182, and miR-183) were examined in 70 paired samples from lung cancer patients (primary cancer and non-cancerous tissues and sera), as well as 44 serum samples from normal volunteers and lung cancer cell lines by quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR). The correlation between the expression of miRNAs in tissues, sera, and patient overall survival were also examined by log-rank and Cox regression analysis.ResultsExpression levels of members of the miR-183 family in lung cancer tumor and sera were higher than that of their normal counterparts. The miR-96 expression in tumors was positively associated with its expression in sera. Log-rank and Cox regression analyses demonstrated that high expression of tumor and serum miRNAs of the miR-183 family were associated with overall poor survival in patients with lung cancer.ConclusionsOur results suggest that the expressions of miR-96, miR-182, and miR-183 in tumor and sera may be considered potential novel biomarkers for the diagnosis and prognosis of lung cancer.

Highlights

  • Lung cancer is the leading cause of cancer-related deaths worldwide

  • Expression of the miR-183 family of miRNAs in lung tissues, sera, and cell lines MicroRNA arrays were performed from 6 lung cancer and 6 matched adjacent normal lung tissues that contained 723 human miRNA probes

  • We selected for further study 3 cluster miRNAs, namely miR-96, miR-182, and miR-183, based on the magnitude of fold changes (10.77, 10.75, and 6.62-fold, respectively) and probability values (P = 0.00127, 0.00209 and 0.00190), as shown in our previous study [24]

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Summary

Introduction

Lung cancer is the leading cause of cancer-related deaths worldwide. MicroRNAs (miRNAs) have shown promise as diagnostic and prognostic indicators. This study was to identify specific miRNAs with diagnostic and prognostic value for patients with lung cancer, and to explore the correlation between expression profiles of miRNAs and patient survival. Lung cancer is the leading cause of cancer-related deaths worldwide [1], and the current pathologic staging is inadequate to predict outcome for patient treatment. We performed a miRNA expression array by quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) and identified the expression of members of the miR-183 family (miR-96, miR182, and miR-183) when comparing primary NSCLC tumor with adjacent normal lung tissues. The role that these miRNAs play in the diagnosis and prognosis of lung cancer patients remains unknown

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