Abstract
Probiotics have evoked great interest in the past years for their beneficial effects. The aim of this study was to investigate whether luxS overexpression promotes the stress resistance of Lactobacillus paraplantarum L-ZS9. Here we show that overexpression of luxS gene increased the production of autoinducer-2 (AI-2, quorum sensing signal molecule) by L. paraplantarum L-ZS9. At the same time, overexpression of luxS promoted heat-, bile salt-resistance and biofilm formation of the strain. RNAseq results indicated that multiple genes encoding transporters, membrane proteins, and transcriptional regulator were regulated by luxS. These results reveal a new role for LuxS in promoting stress resistance and biofilm formation of probiotic starter.
Highlights
Over the past three decades, probiotic potential of some bacteria has been well-recognized
We examined whether luxS overexpression enhances stress resistance of L. paraplantarum L-ZS9 and which genes are regulated by luxS overespression
Recombinant strains luxS-pMG76e-L-ZS9 and pMG76e-L-ZS9 were constructed from parent strain L. paraplantarum L-ZS9 and identified by PCR (Figure 1)
Summary
Over the past three decades, probiotic potential of some bacteria has been well-recognized. The beneficial effects of probiotic bacteria are mainly associated with the maintenance of a healthy gut microbiota, an improvement of gut resilience, the modulation of lactose intolerance, bowel function, and gastrointestinal (GI) comfort, and diarrhea prevention and symptom alleviation (Licciardi et al, 2010; Waitzberg et al, 2015; Wright et al, 2015; Kich et al, 2016; Mokoena et al, 2016). It is important to enhance the stress resistance of probiotic bacteria. Traditional researches focus on the development of protection materials and high density fermentation (Mamvura et al, 2011; Gbassi and Vandamme, 2012; Ortakci et al, 2012; D’Orazio et al, 2015; Ilango et al, 2016; Champagne et al, 2017), there is a great prospect to promote the stress resistance of probiotic bacteria by genetic engineering based on their molecular physiology
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