Over-expression of long noncoding RNA BANCR inhibits malignant phenotypes of human bladder cancer.

Anbang He,Mingwei Chen,Zhaojie Lv,Yuchen Liu,Zhicong Chen,Hongbing Mei,Li Liu,Junhao Lin,Weiren Huang,Jianfa Li,Xinhui Liao,Chengle Zhuang,Yonghao Zhan

doi:10.1186/s13046-016-0397-9

Abstract

BackgroundAccumulating evidences indicated that lncRNAs play crucial regulatory roles in oncogenesis and progression of cancers. BRAF activated non-coding RNA (BANCR) has been identified to contribute to the progression of some human cancers. However, the relationship between BANCR and bladder cancer (BC) is largely unclear.MethodsBANCR expression levels in BC, paired non-cancer tissues and BC cell lines were detected by real-time quantitative RT-PCR (qRT-PCR). The relationships between BANCR expression levels and the clinical characteristics were evaluated. BANCR expression was enhanced by transfecting a pcDNA-BANCR vector. We used both CCK-8 assay and Edu assay to detect cell proliferation. We also detect cell apoptosis and migration by using ELISA assay, Flow cytometry and transwell assay, respectively. All statistical analyses were executed by using the SPSS 20.0 software.ResultsBANCR expression levels were remarkably decreased in BC tissues compared with adjacent noncancerous tissues. BANCR expression levels in two BC cell lines were also significantly down-regulated. Clinicopathologic analysis revealed that low BANCR expression was positively correlated with TNM stage, but not associated with other clinicopathological characteristics. BANCR has been successfully overexpressed in BC cell lines (T24 and SW780) by transfecting a pcDNA-BANCR vector. Cell proliferation inhibition, apoptosis induction and migration suppression were also observed in pCDNA-BANCR-transfected T24 and SW780 cells.ConclusionsThese data suggested that BANCR represents a tumor suppressor player in bladder cancer, contributes to tumor proliferation, apoptosis and migration, and may serve as a new candidate biomarker and a potential therapeutic target for patients with BC.

Highlights

Accumulating evidences indicated that long noncoding RNAs (lncRNAs) play crucial regulatory roles in oncogenesis and progression of cancers
BRAF activated non-coding RNA (BANCR), a 693-nt lncRNA encoded on human chromosome 9, has been found to be aberrant expressed in quite a few cancers, such as colorectal cancer [10], retinoblastoma [11], melanoma [12], papillary thyroid carcinoma [13], lung carcinoma [14],gastric cancer [15] and hepatocellular carcinoma [16]
BANCR was down-regulated in bladder cancer tissues and cells The relative expression level of BANCR was measured by using quantitative RT-PCR (qRT-PCR) in bladder cancer tissues and pair-matched adjacent normal bladder tissues from 54 bladder cancer patients

Summary

Introduction

Accumulating evidences indicated that lncRNAs play crucial regulatory roles in oncogenesis and progression of cancers. With the rapid development of second-generation sequencing technology, a multitude of long noncoding RNAs (lncRNAs) have been found to be dysregulated and involved in the development of various human. He et al Journal of Experimental & Clinical Cancer Research (2016) 35:125 diseases, in cancers [6,7,8]. BRAF activated non-coding RNA (BANCR), a 693-nt lncRNA encoded on human chromosome 9, has been found to be aberrant expressed in quite a few cancers, such as colorectal cancer [10], retinoblastoma [11], melanoma [12], papillary thyroid carcinoma [13], lung carcinoma [14],gastric cancer [15] and hepatocellular carcinoma [16]. Further experiments indicated that the overexpression of lncRNA BANCR could inhibit proliferation, induce apoptosis and suppress migration of the bladder cancer cell lines

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Results

Conclusion