Overexpression of long intergenic noncoding RNA LINC00312 inhibits the invasion and migration of thyroid cancer cells by down-regulating microRNA-197-3p.

Kai Liu,Xiang Min,Wen Huang,Dan-Qing Yan,Qing Luo

doi:10.1042/bsr20170109

Kai Liu, Xiang Min + Show 3 more

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https://doi.org/10.1042/bsr20170109

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Abstract

The study evaluated the ability of long intergenic noncoding RNA LINC00312 (LINC00312) to influence the proliferation, invasion, and migration of thyroid cancer (TC) cells by regulating miRNA-197-3p. TC tissues and adjacent normal tissues were collected from 211 TC patients. K1 (papillary TC), SW579 (squamous TC), and 8505C (anaplastic TC) cell lines were assigned into a blank, negative control (NC), LINC00312 overexpression, miR-197-3p inhibitors, and LINC00312 overexpression + miR-197-3p mimics group. The expression of LINC00312, miR-197-3p, and p120 were measured using quantitative real-time PCR (qRT-PCR) and Western blotting. Cell proliferation was assessed via CCK8 assay, cell invasion through the scratch test, and cell migration via Transwell assay. In comparison with adjacent normal tissues, the expression of LINC00312 is down-regulated and the expression of miR-197-3p is up-regulated in TC tissues. The dual luciferase reporter gene assay confirmed that P120 is a target of miR-197-3p. The expression of LINC00312 and p120 was higher in the LINC00312 overexpression group than in the blank and NV groups. However, the expression of miR-197-3p was lower in the LINC00312 overexpression group than in the blank and NC groups. The miR-197-3p inhibitors group had a higher expression of miR-197-3p, but a lower expression of p120 than the blank and NC groups. The LINC00312 overexpression and miR-197-3p inhibitor groups had reduced cell proliferation, invasion and migration than the blank and NC groups. These results indicate that a LINC00312 overexpression inhibits the proliferation, invasion, and migration of TC cells and that this can be achieved by down-regulating miR-197-3p.

Highlights

Thyroid cancer (TC) accounts for only 1% of all the malignancies and is a common endocrine malignancy, which is derived from follicular thyroid or parafollicular C cells [1]
The expression levels of LINC00312 and miR-197-3p in TC tissues and adjacent normal tissues were detected via quantitative real-time PCR
Death rates associated with TC have declined in most parts of the world, TC incidence has been continuously rising over the recent decades [19]

Summary

Introduction

Thyroid cancer (TC) accounts for only 1% of all the malignancies and is a common endocrine malignancy, which is derived from follicular thyroid or parafollicular C cells [1]. In China in 2000, the incremental incidence and mortality rates of TC in patients below 74 years were 0.32 and 0.03%, respectively [2]. The overall 10-year survival rate of differentiated TC is above 80%, 5–20% of patients develop local or regional recurrences and 10–15% develop distant metastases [3]. Conventional surgery such as adjuvant ablation by radioiodine treatment has been the main therapy for TC. Researchers have attempted to c 2017 The Author(s).

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