Abstract
Age-related macular degeneration (AMD) mainly affects the central region of retina and has many late-stage manifestations. Age-related macular degeneration is a leading cause of irreversible blindness in older people. The main feature of AMD is retinal pigment epithelium (RPE) degeneration. In this study, we aimed to explore the influence of HTRA1 expression on the proliferation and migration of RPE cells. Human ARPE-19 cells were transfected with an HTRA1 overexpression lentivirus or HTRA1 siRNA to silence HtrA1 expression. Quantitave reverse-transcription polymerase chain reaction (qRT-PCR) and western blotting were used to verify the relative level of HTRA1 mRNA and expression of HTRA1 protein of transfected human ARPE-19 cells. The MTT clone formation and transwell assays were used to confirm the effect of HTRA1 expression on the proliferation, colony forming ability and migration of ARPE-19 cells. The proliferation capacity (shown as optical density value) of ARPE-19 cells in the HTRA1-overexpressing group at culture times of 24 h and 48 h were 0.595 ±0.032 and 0.867 ±0.037 respectively, which were much higher than in the mock group. However, the proliferative capacity of cells in the HTRA1-silenced group decreased with increasing time of culture, compared with the mock group. The number of cloned and migrating cells in the HTRA1-overexpressing group were much higher than in the mock group, whereas the numbers in the HTRA1-silenced group were significantly lower. Overexpression of HTRA1 promotes proliferation and migration of RPE cells, which can help maintain the function of sensory neurons in the retina. Therefore, HTRA1 may be a suitable target for AMD treatments.
Highlights
Age-related macular degeneration (AMD) mainly affects the central region of retina and has many late-stage manifestations
We aimed to explore the influence of HtrA serine peptidase 1 (HTRA1) expression on the proliferation and migration of retinal pigment epithelium (RPE) cells
Overexpression of HTRA1 promotes proliferation and migration of RPE cells, which can help maintain the function of sensory neurons in the retina
Summary
Age-related macular degeneration (AMD) mainly affects the central region of retina and has many late-stage manifestations. Age-related macular degeneration (AMD) is an eye disease that mainly affects the central region of retina with many late-stage manifestations.[1] An abundance of cone photoreceptors in the retina can impact visual acuity. The unusual structure of the RPE is closely related to the etiology of AMD.[3,4] Previous data have shown that age-related maculopathy susceptibility 2 (ARMS2) and HtrA serine peptidase 1 (HTRA1) located at chromosome 10q26 are closely associated with susceptibility to AMD.[5,6] Wang et al speculated that HTRA1 might be an important risk factor in AMD.[7]
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