Overexpression of Hepatocyte Growth Factor and Its Receptor c-Met in Nasal Polyps

Abstract

To investigate the expression and distribution of hepatocyte growth factor (HGF) and c-Met proteins in normal nasal mucosa and in nasal polyps and to evaluate the possible effects of HGF and c-Met on the development of nasal polyps. Prospective study. Tertiary academic institution. Normal inferior turbinate mucosa was obtained from 20 patients undergoing surgery for augmentation rhinoplasty. Nasal polyps were obtained from 20 patients undergoing endoscopic sinus surgery for chronic polypoid sinusitis. Semiquantitative reverse-transcriptase polymerase chain reaction, immunohistochemistry, and Western blot analysis were performed. The expression and distribution of HGF and c-Met were analyzed. Using immunohistochemistry, moderate to high levels of HGF were mainly localized in submucosal glands of nasal polyps, while c-Met was detected in submucosal glands and in epithelial cells. In normal turbinate mucosa, immunopositive HGF was detected in submucosal glands, where faint staining was found, while c-Met was noted in epithelial cells and in submucosal glands. Semiquantitative reverse-transcriptase polymerase chain reaction and Western blot analysis showed that HGF expression was increased in nasal polyps compared with that in normal turbinate mucosa. The same result was observed for c-Met. Elevated c-Met expression in combination with expression of HGF in nasal polyps may enhance the proliferation of epithelial cells and submucosal glandular cells through the release of HGF, which activates c-Met receptors in nasal polyps.