Abstract
The flowering time and plant height of soybean are important agronomic characters, which control the adaptability and yield of soybean. R2R3 MYB transcription factor plays an important regulatory role in plant growth and development. In this study, soybean GmGAMYB gene of R2R3-MYB type was induced by long-days (LDs). GmGAMYB showed higher transcriptional levels in the flowers, leaves and pods of soybean. Overexpression of GmGAMYB in transgenic soybean showed earlier flowering time and maturity in LDs and short-days (SDs). GmGAMYB interacted with GmGBP1 and might promote flowering time by up-regulating the expression of GmFULc gene in soybean. Moreover, the expression level of GmGAMYB was also induced by gibberellins (GAs) and the plant height of GmGAMYB-ox plants was significantly increased, which was caused by the enlargement of internode cell in stem. Furthermore, GmGAMYB overexpression led to increased GA sensitivity in the hypocotyl of soybean seedlings compared with WT. GmGAMYB may be a positive regulator of GA response of promoting plant height by up-regulating the expression of GmGA20ox gene in soybean. Together, our studies preliminarily showed that the partial functions of GmGAMYB in regulating flowering time and GA pathway.
Highlights
IntroductionMaturity and plant height are the key factors affecting soybean adaptability and yield
Soybean flowering time, maturity and plant height are the key factors affecting soybean adaptability and yield
Multiple sequence alignment of soybean GmGAMYB and GAMYB-like proteins of Arabidopsis thaliana, Oryza sativa, Zea mays, Vitis vinifera, Manihot esculenta, Solanum lycopersicum, and Cucumis sativus showed that GmGAMYB protein contains a highly conserved R2R3 domain in the N-terminal (Figure 1A), which is the typical feature of R2R3-MYB subfamily
Summary
Maturity and plant height are the key factors affecting soybean adaptability and yield. SD can promote flowering, and longday (LD) inhibit the growth of flower bud (Kantolic and Slafer, 2007). This characteristic seriously hinders the adaptability of soybean varieties, and some soybean varieties planted in areas beyond their normal latitude of 2◦N may significantly reduce their yields (Gai and Wang, 2001), so different types of varieties in photoperiod response are needed to adapt to different ecological conditions. GmAP1, a AP1 homologous gene in soybean, promotes early flowering and the alteration of floral organ patterns in tobacco (Chi et al, 2011)
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