Abstract
AimsFar upstream element-binding protein 1 (FUBP1) has been shown to involve in the tumorigenesis and tumor progression of various cancers. However, the expression and function of FUBP1 in cervical carcinoma remains unknown. Main methodsTranscriptional expression of FUBP1 was initially evaluated using the Oncomine database, followed by evaluation of FUBP1 protein levels using immunohistochemistry in 119 cervical carcinoma patient tissues. In vitro experiments were performed to assess the tumorigenic role of FUBP1. Besides, Gene Set Enrichment Analysis, EnrichmentMap analysis, and protein-protein interaction (PPI) networks were used to evaluate the potential mechanisms of FUBP1 in promoting cervical cancer progression. Key fundingsIn this research, we found both FUBP1 mRNA transcription and protein expression levels increased significantly in cervical carcinoma tissues compared with adjacent normal cervical tissues. Furthermore, elevated FUBP1 expression was positively correlated with age, T classification, N classification, tumor recurrence, Ki67 expression, and poor prognosis in cervical carcinoma patients. Besides, elevated FUBP1 expression acted as an independent unfavorable predictor for overall survival and disease-free survival in cervical carcinoma. Overexpression of FUBP1 significantly promoted cervical carcinoma cell proliferation and inhibits cell apoptosis in vitro, while knockdown of FUBP1 showed the opposite effect. Mechanistically, bioinformatics analysis revealed that FUBP1 promoted the biological function of cervical carcinoma cells via enhancing DNA repair signal pathways. Our results demonstrate for the first time that FUBP1 is a novel prognostic factor and therapeutic target for cervical carcinoma.
Highlights
Far upstream element-binding protein 1 (FUBP1) has been shown to involve in the tumorigenesis and tumor progression of various cancers
To explore the expression of FUBP1 in cervical carcinoma, we firstly analyzed the mRNA expression of FUBP1 in the normal Cervix Uteri tissues and cervical carcinoma tissues through the Oncomine database (Fig. 1A)
To further confirm whether the protein levels of FUBP1 change, we detected the expression of FUBP1 in a cervical carcinoma Tissue Microarray by IHC staining
Summary
Far upstream element-binding protein 1 (FUBP1) has been shown to involve in the tumorigenesis and tumor progression of various cancers. The expression and function of FUBP1 in cervical carcinoma remains unknown. As the fourth common malignancy and the fourth leading cause of death in women, cervical carcinoma is one of the most prominent gynecologic malignancies among women worldwide, especially in developing countries[1, 2]. It is reported that about 530,000 female patients are newly diagnosed, and approximately 275,000 patients die of cervical carcinoma every year[3]. With the widespread application of thin-preparation cytologic test and human papillomavirus prophylactic vaccine, the incidence rate of cervical carcinoma in developed countries is decreasing[4]. Unlike breast cancer and colorectal cancer, the significant molecular therapeutic targets have not been identified clearly in cervical carcinoma[6, 7]. It is necessary to clarify the underlying pathogenesis and provide molecular targeted therapy in cervical carcinoma
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