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Abstract
Drought is the primary disaster that endangers agricultural production, including animal husbandry, and affects the distribution, growth, yield, and quality of crops. Previous study had revealed that DIP, as a potential regulator of DBF activity, played an important role in response to drought stress in maize. In this study, a total of 67 DIPs were identified from seventeen land plants, including six tobacco DIPs (NtDIPs). NtDIP6 gene was further selected as a candidate gene for subsequent experiments based on the phylogenetic analysis and structural analysis. The transgenic tobacco and poplar plants over-expressing NtDIP6 gene were generated using the Agrobacterium- mediated method. Although there was not phenotypic difference between transgenic plants and wild-type plants under normal conditions, overexpression of the NtDIP6 gene in transgenic tobacco and poplar plants enhanced the drought tolerance under drought treatments in comparison with the wild type. The content of antioxidant defense enzymes peroxidase (POD), catalase (CAT), and the photosynthetic rate increased in NtDIP6-Ox transgenic tobacco and poplar plants, while the content of malondialdehyde decreased, suggesting that the overexpression of NtDIP6 enhances the antioxidant capacity of transgenic poplar. Furthermore, the results of qRT-PCR showed that the level of expression of drought-related response genes significantly increased in the NtDIP6-Ox transgenic plants. These results indicated that NtDIP6, as a positive response regulator, improves drought stress tolerance by scavenging superoxide via the accumulation of antioxidant defense enzymes.
Highlights
As sessile organisms, the growth and development of plant is severely restricted by environmental stresses, such as drought, high salinity, and high and low temperatures
DBF1 and DBF2 are involved in rab17 regulation through the drought-responsive element in an ABA-dependent pathway
NtDIP3 and NtDIP4 were on chromosome 11; NtDIP1 was on chromosome 24, and NtDIP2, 5, 6 were on three scaffolds (Nitab4.5_0001437, Nitab4.5_0009285, and Nitab4.5_0011086), respectively
Summary
The growth and development of plant is severely restricted by environmental stresses, such as drought, high salinity, and high and low temperatures. Recent studies revealed that gene expression, transcriptional regulation, and signal transduction are involved in the regulation of responses of plants to drought (Zhu et al, 2010). DBF (Dehydration responsive element binding factor), as a transcription factor, was introduced by Kizis and Pages (2002), activates drought stress tolerance genes in many plants. The DBF is a part of the Apetala 2/Ethylene Response Factor (AP2/ERF) transcription factor family and induces the rab (responsive to abscisic acid) gene expression under drought stress conditions (Kizis and Pages, 2002). DBF1 and DBF2 are involved in rab regulation through the drought-responsive element in an ABA-dependent pathway. Xu et al (2008) identified three new DBF genes in T. aestivum (named TaAIDFs, T. aestivum abiotic stress-induced DBFs) by screening a wheat cDNA library after drought treatment
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