Abstract
The aim of this study was to evaluate expression of COX-1 in renal cell carcinoma (RCC) and its prognostic value. mRNA of COX-1 was detected in 42 paired RCC and adjacent normal tissues with quantitative real- time polymerase chain reaction (qRT-PCR). Expression of COX-1 was also evaluated in 196 RCC sections and 91 adjacent normal tissues with immunohistochemistry. Statistical analysis was performed to assess COX-1 expression in RCC and its prognostic significance. The results of qRT-PCR showed mRNA levels of COX-1 in RCC tissues to be significantly higher than that in adjacent normal tissues (p < 0.001). Immunohistochemical assays also revealed COX-1 to be overexpressed in RCC tissues (p < 0.001). Statistical analysis demonstrated high expression of COX-1 was correlated with tumour size (p = 0.002), pathological stage (p = 0.003), TNM stage (p = 0.003, 0.007, 0.027, respectively), and tumour recurrence (p < 0.001). Survival analysis indicated patients with high expression of COX-1 had shorter survival time (p < 0.001), and COX-1 was an independent predictor. This is the first study to reveal overexpression of COX-1 in RRC and point to use as a prognostic marker in affected patients.
Highlights
Renal cell carcinoma (RCC) is the second most common tumor in urinary system, accounting for 3% of adult malignant tumor (Jemal et al, 2010; Siegel et al, 2012)
The aim of this study was to evaluate expression of COX-1 in renal cell carcinoma (RCC) and its prognostic value. mRNA of COX-1 was detected in 42 paired RCC and adjacent normal tissues with quantitative realtime polymerase chain reaction
About 30% of patients have metastatic disease when diagnosed with RCC and radical nephrectomy remains the main treatment for RCC patients because of the tumor’s resistance to radiation and chemotherapy
Summary
Renal cell carcinoma (RCC) is the second most common tumor in urinary system, accounting for 3% of adult malignant tumor (Jemal et al, 2010; Siegel et al, 2012). Approximately 30% of RCC patients experience local or distant recurrence after radical nephrectomy (Skinner et al, 1971; Patel et al, 2012). Some studies have reported that COX-1 was over expressed in epithelial ovarian cancer and it was the major prostanoid generating pathway operative in ovarian cancers of epithelial origin (Gupta et al, 2003; Daikoku et al, 2005). There have been quite a few studies on the role of COX-2 in RCC, but the action of COX-1 in RCC is still unclear up to present. The aim of present study was, to investigate the expression of COX-1 in normal human kidney and in different types of RCC, and to test its
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