Abstract
As the group II LEA (late embryogenesis abundant) proteins, dehydrins (DHNs) play an important role in plant growth and development, as well as in response to abiotic or biotic stress challenges. In this study, a DHN gene named CsLEA11 was identified and characterized from Cucumis sativus. Sequence analysis of CsLEA11 showed that it is a Y3SK2-type DHN protein rich in hydrophilic amino acids. Expression analyses revealed that the transcription of CsLEA11 could be significantly induced by heat and cold stress. The recombinant plasmid was transformed into Escherichia coli BL21 and isopropy-β-d-thiogalactoside (IPTG) was used to induce recombinant E. coli to express CsLEA11 gene. Overexpression of CsLEA11 in E. coli enhanced cell viability and conferred tolerance to heat and cold stress. Furthermore, CsLEA11 protein could protect the activity of lactate dehydrogenase (LDH) under heat stress. Taken together, our data demonstrate that CsLEA11 might function in tolerance of cucumber to heat and cold stress.
Highlights
Temperature is one of the major factors affecting plant growth and productive capacity (Guo et al 2017)
late embryogenesis abundant (LEA) proteins are synthesized in large amounts during the late stages of seed development, enabling the maturing seeds to acquire desiccation tolerance, which plays an important role in the adaptation of plants to abiotic stresses (Hu et al 2016; Tang et al 2016; Tolleter et al 2007)
The CsLEA11 protein was rich in hydrophilic amino acids such as Thr (13.0%), Gly (10.5%), Ser (9.3%), Glu (8.6%), Lys (8.0%), and His (7.4%), but was lack of the hydrophobic amino acids Cys and Trp
Summary
Temperature is one of the major factors affecting plant growth and productive capacity (Guo et al 2017). Wheat WZY2 can act as a protectant to increase Escherichia coli viability, protect lactate dehydrogenase (LDH) activity and inhibit protein aggregation during temperature variation including heat and cold (Yang et al 2015). A number of studies have shown that the cold stress tolerance was enhanced by overexpression of different DHNs in various plant species, such as Arabidopsis (Aguayo et al 2016; Ochoa-Alfaro et al 2012; Peng et al 2008), tobacco (Guo et al 2017; Hill et al 2016; Liu et al 2014; Xing et al 2011), strawberry (Houde et al 2004), cucumber (Yin et al 2006), and tomato (Liu et al 2015), suggesting their possible functions in cold stress. Silencing of either CaDHN1 or CaDHN3 in pepper resulted in obviously lower resistance to cold stress compared with the control (Chen et al 2015; Jing et al 2016)
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