Abstract
BackgroundContactin1 (CNTN1) has been shown to play an important role in the invasion and metastasis of several tumors; however, the role of CNTN1 in breast cancer has not been fully studied. The purpose of this study is to investigate the role of CNTN1 in regulating tumor growth, migration and invasion in breast cancer.ResultsTo investigate its function, CNTN1 was expressed in Hs578T cells. CNTN1 expression was confirmed by western blot, immunohistochemistry and real-time RT-PCR. The effect of CNTN1 overexpression on proliferation, migration and invasion of Hs578T breast cancer cells was assessed in vitro and in vivo. Our results showed that CNTN1 overexpression promoted Hs578T cell proliferation, cell cycle progression, colony formation, invasion and migration. Notably, overexpression of CNTN1 in Hs578T cells enhanced the growth of mouse xenograft tumors.ConclusionsCNTN1 promotes growth, metastasis and invasion of Hs578T breast cancer cell line. Thus, therapies targeting CNTN1 may prove efficacious for breast cancer. However, further investigation is required to understand the mechanism by which CNTN1 influences proliferation, metastasis and invasion in breast cancer.
Highlights
Contactin1 (CNTN1) has been shown to play an important role in the invasion and metastasis of several tumors; the role of CNTN1 in breast cancer has not been fully studied
In hepatocellular carcinoma (HCC) tissues, CNTN1 overexpression was reported to be closely associated with aggressive clinicopathological features, suggesting that CNTN1 may be involved in tumor metastasis and invasion in HCC [12]
Expression of CNTN1 in breast cancer cells We first measured the expression of CNTN1 in several breast cancer cell lines (MCF7-ADR, MDA-MB-468, MCF7 and Hs578T) using reverse transcriptase polymerase chain reaction (RT-polymerase chain reaction (PCR)) and western blotting
Summary
Contactin (CNTN1) has been shown to play an important role in the invasion and metastasis of several tumors; the role of CNTN1 in breast cancer has not been fully studied. The purpose of this study is to investigate the role of CNTN1 in regulating tumor growth, migration and invasion in breast cancer. Breast cancer mortalities are usually associated with spread and metastasis. CNTN1 is abundant in the human brain and neural tissues, Chen et al BMC Cell Biology (2018) 19:5 mediator of VEGF-C-induced migration in esophageal cancer. In hepatocellular carcinoma (HCC) tissues, CNTN1 overexpression was reported to be closely associated with aggressive clinicopathological features, suggesting that CNTN1 may be involved in tumor metastasis and invasion in HCC [12]
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