Overexpression of centromere protein K (CENPK) in ovarian cancer is correlated with poor patient survival and associated with predictive and prognostic relevance.

Yi-Chao Lee,Ding-Yen Lin,Chi-Chen Huang,Wen-Chang Chang,Kuen-Haur Lee

doi:10.7717/peerj.1386

Yi-Chao Lee, Ding-Yen Lin + Show 3 more

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https://doi.org/10.7717/peerj.1386

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Journal: PeerJ	Publication Date: Nov 5, 2015
Citations: 36	License type: CC BY 4.0

Affiliation: Taipei Medical University, National Cheng Kung University

Abstract

Ovarian cancer has a poor prognosis. Most patients are diagnosed with ovarian cancer when the disease has reached an advanced stage and cure rates are generally under 30%. Hence, early diagnosis of ovarian cancer is the best means to control the disease in the long term and abate mortality. So far, cancer antigen 125 (CA125) and human epididymis protein 4 (HE4) are the gold-standard tumor markers for ovarian cancer; however, these two markers can be elevated in a number of conditions unrelated to ovarian cancer, resulting in decreased specifically and positive predictive value. Therefore, it is urgent to identify novel biomarkers with high reliability and sensitivity for ovarian cancer. In this study for the first time, we identified a member of the centromere protein (CENP) family, CENPK, which was specifically upregulated in ovarian cancer tissues and cell lines and the overexpression of which was associated with poor prognoses in patients with ovarian cancer. In addition, the presence of CENPK significantly improved the sensitivity of CA125 or HE4 for predicting clinical outcomes of ovarian cancer patients. In conclusion, we identified that CENPK was specifically upregulated in ovarian cancer cells and can be used as a novel tumor marker of ovarian cancer.

Highlights

Ovarian cancer is the most lethal gynecologic malignancy in women, with 21,290 estimated new cases and 14,180 estimated deaths in 2014 in the US alone (Siegel, Miller & Jemal, 2015)
centromere protein K (CENPK) was overexpressed in various human cancers To understand gene expression levels of centromere protein (CENP) family in various human cancers, the mRNA expression of levels of CENP family were analyzed by using Cancer Genome Anatomy Project (CGAP) gene expression database
Compared to three non-tumorigenic cell lines (H184B5H5/M10, T/G HA-VSMC, and HFL1), CENPK mRNA was highly expressed in ovarian cancer cell lines, including TOV-21G, OC314, and TOV-112D (Fig. 2A)

Summary

Introduction

Ovarian cancer is the most lethal gynecologic malignancy in women, with 21,290 estimated new cases and 14,180 estimated deaths in 2014 in the US alone (Siegel, Miller & Jemal, 2015). In the early stages of ovarian cancer, no symptoms are evident, or symptoms are similar to other benign gynecological diseases (Bast, Hennessy & Mills, 2009). Most of these tumors are detected at an advanced stage ( in stage III) with. How to cite this article Lee et al (2015), Overexpression of centromere protein K (CENPK) in ovarian cancer is correlated with poor patient survival and associated with predictive and prognostic relevance. Identification and validation of specific novel biomarker for diagnosing ovarian cancer is the best means to control the disease in the long term and abate mortality (Rauh-Hain et al, 2011)

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