Abstract

To express the cbhI gene, encoding Aspergillus aculeatus cellobiohydrolase I (CBHI), in Aspergillus oryzae, a plasmid was constructed. The strain that displayed the strongest CBHI activity among the transformants produced about 941 mg/ l in liquid culture. It was confirmed by a PCR method that the plasmid was integrated at the niaD locus.

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