Abstract
Peanut (Arachis hypogaea L.) is one of the most susceptible host crops to Aspergillus flavus invasion and subsequent aflatoxin contamination. In this report, a new member of PR10 family putative resistant gene (designated as ARAhPR10, No. EU661964.1) encoding a PR10 protein was isolated and characterized. Analysis of qRT-PCR showed that the expression of ARAhPR10 was induced by pre-harvested A. flavus infection, but no significant difference was observed between resistant genotype “GT-C20” and susceptible genotype “Yueyou 7”. Seven transgenic peanut lines expressing the ARAhPR10 gene under the control of 35S promoter were obtained using the Agrobacterium tumefaciens-mediated method. Real time RT-PCR results showed that the expression level of the ARAhPR10 was significantly higher and the A. flavus infection and aflatoxin content were significantly lower in seeds of transgenic lines than that of the wild type. A significant negative correlation between ARAhPR10 expression at transcript level and seeds aflatoxin production was observed. Combining the previous results, it is suggested that ARAhPR10 expression play an important role in peanut host resistance to A. flavus infection and aflatoxin producing.
Highlights
Peanut is one of the most susceptible host crops to Aspergillus flavus invasion and subsequent aflatoxin production
It is suggested that ARAhPR10 expression play an important role in peanut host resistance to A. flavus infection and aflatoxin producing
To validate whether the expression of the ARAhPR 10 genes had relationship with pre-harvested A. flavus infection, total RNA were extracted from pods of resistant cultivar “CT-20” and the susceptible cultivar “Yueyou7” under well-watered, drought stress and A. flavus infection accompanied with drought stress on the 30th days after treatments, and the expression levels of ARAhPR10 in resistant and susceptible genotypes were analyzed through real time RT-PCR (Figure 2)
Summary
Peanut is one of the most susceptible host crops to Aspergillus flavus invasion and subsequent aflatoxin production. With the application of proteomics and genomics technology to high-throughput gene identification, many resistance-associated proteins and genes have been identified in maize [4,5,6] and peanut [7,8,9]. These proteins and genes comprise seed storage proteins, antifungal proteins, pathogenesis-related proteins and stress-related proteins [4,5,6,7,8,9]. The role of PR10 in host resistant to aflatoxin received more attention
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