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Abstract
Objective To investigate the role of aquaglyceroporin 7 (AQP7) played in insulin resistance of adipocytes.Methods 3T3-L1 preadipocyte cells were induced to be fully differentiated adipocytes and then insulin resistance was induced by dexamethasone (DXM).Adenovirus vector over-expressing AQP7 (Ad-AQP7) was constructed and transfected into adipocytes.Expression levels of AQP7,glycerol release from adipocytes,changes in glucose consumption,and phosphorylated protein kinase B (p-PKB) were measured.Results 48 h-treatment of dexamethasone significantly inhibited the expression level of p-PKB (P＜0.01) and decreased glucose utilization,as well as down-regulation of AQP7 (P＜0.01).Over-expression of AQP7 by transfecting Ad-AQP7 to dexamethasone (DXM)-induced insulin resistant adipocytes could improve glycerol secretion of adipocytes,being consistent with the restoration of p-PKB expression levels and glucose consumption.Conclusions AQP7 may improve in molecular mechanism of insulin resistance in adipocytes.Overexpression of AQP7 may contribute to the improvement of insulin resistance in adipocytes and might be correlated with better phosphorylation of PKB. Key words: Aquaglyceroporin 7 ; Insulin resistance ; Protein kinase B
Published Version
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