Abstract
Ectopic expression of the MYB transcription factor of AmROSEA1 from Antirrhinum majus has been reported to change anthocyanin and other metabolites in several species. In this study, we found that overexpression of AmRosea1 significantly improved the tolerance of transgenic rice to drought and salinity stresses. Transcriptome analysis revealed that a considerable number of stress-related genes were affected by exogenous AmRosea1 during both drought and salinity stress treatments. These affected genes are involved in stress signal transduction, the hormone signal pathway, ion homeostasis and the enzymes that remove peroxides. This work suggests that the AmRosea1 gene is a potential candidate for genetic engineering of crops.
Highlights
Anthocyanins are pigments that belong to the flavonoid class and are synthesized via the phenylpropanoid pathway, which are responsible for many colors, such as red, purple, and blue in leaves, flowers, and fruits of angiosperms
The expression of flavanone 3-hydroxylase (F3H), flavonol synthase (FLS), flavonoid 3’-hydroxylase (F3 H), dihydroflavonol 4-reductase (DFR), leucoanthocyanidin dioxygenase (LDOX), and UDP-glucose 3-O-flavonoid transferase (UFGT) genes are highly dependent on AmROSEA1
We found that AmROSEA1 could not effectively induce anthocyanin biosynthesis in rice, but clearly improved the tolerance of transgenic rice to drought and salinity stresses
Summary
Anthocyanins are pigments that belong to the flavonoid class and are synthesized via the phenylpropanoid pathway, which are responsible for many colors, such as red, purple, and blue in leaves, flowers, and fruits of angiosperms. AtMYB2 protein up-regulated the expression of ABA-inducible genes in drought-treated Arabidopsis plants [14], and overexpression of AtMYB15 enhanced the drought tolerance of transgenic Arabidopsis [15]. We used Illumina sequencing technology to analyze the transcriptomes of wild type (WT) and transgenic rice plants under drought and salinity conditions and discovered that the transcriptional level of a considerable number of stress-related genes was affected by exogenous AmRosea. We used Illumina sequencing technology to analyze the transcriptomes of wild type (WT) and transgenic rice plants under drought and salinity conditions and discovered that the transcriptional level of a considerable number of stress-related genes was affected by exogenous AmRosea1 These affected genes were involved in stress signal transduction, the hormone signal pathway, ion balance, and the removal of reactive oxygen species (ROS)
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